University Of Tehran Press Pollution 2383-451X 3 1 2017 01 01 Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03 101 111 59576 10.22059/poll.2017.59576 EN Moslem Papizadeh Microorganisms Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran Mohammad Roayaei Ardakani Biotechnology and Biology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Hossein Motamedi Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran Journal Article 2016 06 28 Petroleum-polluted soil samples from Ahvaz oilfield were enriched, using three methods to detect microorganisms with different dibenzothiophene degradation capabilities. Strain NISOC-03, a nitrate-reducing, oxidase negative, catalase, citrate, and urease positive, gram negative rod, showed interesting dibenzothiophene desulfurization behavior, designated as <em>Entreobacter</em> sp. strain NISOC-03 based on phenotype and genotype analyses. Gas chromatography, biomass measurement, and Gibb’s assay showed that in the presence of benzoate as the carbon source, strain NISOC-03 utilized 64% of 0.8 mM dibenzothiophene, producing 0.27 mM phenyl phenol during the exponential growth phase, though the produced phenyl phenol was degraded in the stationary growth phase. In the presence of glucose as the carbon source, however, strain NISOC-03 metabolized only 19.6% of 0.8 mM dibenzothiophene. Furthermore, replacing glucose with ethanol or glycerol led to the same reduction of the dibenzothiophene utilization. It is thus concluded that the chemistry of the potential carbon source(s) in the culture medium has a significant influence on the quality and the rate of dibenzothiophene metablization, and the enrichment designation has a very vital effect on the biodegradation efficiency of the isolated microorganisms. https://jpoll.ut.ac.ir/article_59576_2953db15d44cd01828cc9da529c3f37a.pdf