Diesel Oil Utilization Efficiency of Selective Bacterial Isolates from Automobile Workshop and Thesjaswini River of Kerala

Document Type : Original Research Paper

Authors

1 Department of Environmental Science, Central University of Kerala, Kerala,India

2 Department of Environmental Science, School of Energy, Environment and Earth Sciences, Central University of Kerala, India

Abstract

Bioremoval and bioreduction activities of hydrocarbon (diesel) isolation from environmental samples were studied by the activity of biosurfactant production, and calculating emulsification index, gravimetric, and FTIR analysis along with the estimation of bacterial biomass. Sample from soil near petrol, diesel pumps and water sample from Thesjaswini River near Padannakad, Kasaragod, Kerala, India, were used to screen the potential diesel oil utilizing bacteria. Among the bacterial isolates (Staphylococcus, Bacillus and Corynebacterium strains), Staphylococcus sp was the potent degraders of diesel oil. Staphylococcus strain was observed to be maximum diesel oil utilizing ability (73% emulsification index) and change in the functional groups of the compound (FTIR analysis). The strain showed optimal growth at 37oC with pH 7, agitation of 150 rpm and time period (5days). The results revealed the possibility to use these strain for the reduction of complex hydrocarbon in ecosystems where they accumulate and cause pollution problems. The highest rate of hydrocarbon degradation occurred when the bacterial strain is a biosurfactants producer. The selective strain produces biosurfactants which increase the interfacial area for contact to give improved uptake of hydrophobic substrates. Bacterial strains capable of degrading complex hydrocarbons, present in the environment, have a potential to be used as an effective tool for removing ecotoxic compounds. Furthermore, results indicated that the bacterial strain Staphylococcus sp could be potentially used in biodegradation of diesel oil in waste water and had a promising application in bioremediation of hydrocarbon contaminated environments.

Keywords


Atlas, R.M. (1995). Petroleum Biodegradation and Oil Spill Bioremediation. Marine. Poll. Bull., 31: 178-182.
Chandran, P. and Das, N. (2010). Biosurfactant production and diesel oil degradation by yeast species Trichosporon asahii isolated from petroleum hydrocarbon contaminated soil. Int. J. Engg .Sci. Technol., 2(12): 6942-6953.
EPA., (2005). An analysis of diesel air pollution and public health in America. Retrieved from EPA United States Environmental Protection Agency. Accessed on 12.3.2015.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. (1994). Bergey’s Manual of Determinative Systematic Bacteriology. Lippincott Williams and Wilkins, A Wolters Kluwer Company, Philadelphia.
Lal, B., Khanna, S. (1996). Degradation of crude oil by Acinetobacterca lcoaceticus and Alcaligenes odorans. J. Appl. Bacteriol., 4: 355-362.
Milic, J.S., Beskoski, V.P., Ilic, M.V., Ali, S.A.M. and Vrvic, M.M. (2009). Bioremediation of soil heavily contaminated with crude oil and its products: compostion of the microbial consortium. J.Serb. Chem. Soc., 74: 455-460.
Olson, J.J., Mills, G.L., Herbert, B.E. and Moris, P.J. (1999). Biodegradation rates of separated diesel components. Environ. Tox. Chem., 18: 2448-2453.
Priya., Sonawani, R., Manjusha and Kulkarni, M. (2014). Bioremediation of soil contaminated with petroleum oil by using Actinomycetes and Bacillus species. Int. J. Pharm. Res. Dev., 6(10):48-52.
Rahman, K.S., Rahman, T., Lakshmanaperumalsamy, P. and Banat, I.M. (2002). Occurrence of crude oil degrading bacteria in gasoline and diesel station soils. J. Basic. Microbiol., 42: 284-291.
Ranjana Julias Thilakar and Jeya Rathi, J. (2013). Bioremediation of diesel contaminated soil by oil degrading bacteria (Pseudomonas sp) using biostimulation method, J. Microbiol. Biotechnol. Res., 3(5): 18-26.
Rodrigues, L.R., Teixeira, J.A., Van der Mei, H.C. and Oliveira, R. (2006). Physiochemical and functional characterization of a biosurfactant produced by Lactococcus lactis 53. Colloid. Surface. B., 49(1): 79 - 86.
Rodriguez-Rodriguez, C., Carolina, Z. and Carolina, B. (2012). Evaluation of growth in diesel fuel and surfactants production ability by bacteria isolated from fuels in Costa Rica. Rev. Soc. Ven. Microbiology 32, 116-120.
Saher, M., Jesse, S.D. and Pattanathu, K.S.M.R. (2011). Influence of nitrogen and phosphorus on rhamnolipidbiosurfactant production by Pseudomonas aeruginosaDS10-129 glycerol as carbon source. Biotechnol., 10(2): 183-189.
Sharma, S.L. and Pant, A. (2001), Crude oil degradation by a marine actinomycete Rodococcus sp. Indian J. Mar. Sci., 4: 245-247.
Varadavenkatesan, T. and Murty, V.R. (2013). Production of a lipopeptidebiosurfactants by a novel Bacillus sp.and its applicability to enhanced oil recovery. Microbiol. J. doi: 10.1155/2013/621519.
Vilma Cipinyte, Saulius Grigiskis., DovilÄ— Sapokaite and Egidijus Baskys (2011). Production of biosurfactants by arthrobacter sp. n3, a hydrocarbon degrading bacterium; Environ. Technol. Resour., 1: 68-75. ISBN 978-9984-44-070-5.
Vinod Kumar, V., Bitan Basu, Ramanathan Ramaswami, SivasubramanianViswanathan., Iyyappan Divya Priya., jayarajan Jeyan and Amala Reddy (2014). Analysis of aromatic hydrocarbon degrading capacity of thermophilic bacteria isolated from contaminated soil. J Chemtech. Res., 6(10): 4556-4563.
Youssef, N.H., Duncan, K.E., Nagle, D.P., Savage, K.N., Knapp, R.M. and Michael, J.M. (2004). Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Meth., 56(3): 339-347.