Mycoremediation of Dichlorvos Pesticide Contaminated Soil by Pleurotus pulmonarius (Fries) Quelet

Document Type : Original Research Paper

Authors

Environmental Biology Research Unit, Cell Biology and Genetics Department, University of Lagos, Akoka, Lagos, Nigeria

Abstract

The extensive use of pesticides leads to accumulation of a huge amount of residues in the environment. As such, the present study investigates the potentiality of Pleurotus pulmonarius to bioremediate dichlorvos pesticides (2,2-dichlorovinyldimethylphosphate) in contaminated soil. DDVP-polluted soils have been contaminated in five concentrations (5% v/w, 10% v/w, 15% v/w, 20% v/w, and 25% v/w), and the soil samples have been inoculated and incubated with pure culture of growing spawns of P. pulmonarius, obtained from commercial mushroom laboratory of Federal Institute of Industrial Research Oshodi, Lagos. The control, however, has not been inoculated. Each treatment has been in triplicates with the soils, analyzed for total amount of DDVP at day 0 and day 60, using gas chromatography and mass spectrometry. Also, pH, moisture content, and total organic matter of the soil have been determined. Results show that the rate of DDVP degradation in the soils with Pleurotus pulmonarius has been higher than the soil samples without mushroom after 60 days. However, for the control without mushroom (loss due to natural attenuation) and those inoculated with P. pulmonarius (bioremediation) the loss percentage of DDVP ascended with the percentage of pesticide from 5% to 25%. The DDVP loss across all different concentrations of mushroom inoculation have been significant (p<0.05); however, for natural attenuation, it has not been significant (p>0.05), except for the lowest pesticide level (5%). Activities of mycelia have decreased soil pH, moisture content, and total organic matter. There has been a very minimal pesticide bioaccumulation in mushroom tissue, which has not been significant (p>0.05), but considerable at p<0.001, indicating that P. pulmonarius has the potential to degrade DDVP pesticides in soil.

Keywords

References

Adelaja, O.D., Njoku, K.L. and Akinola, M.O. (2017) Mycoremediation of Pesticide Contaminated Soil Using Mushroom Pleurotus ostreatus. International Journal of Health and Economic Development, 3(2), 20-27.
Adenipekun, C.O., Ayanleye, O.O. and Oyetunji, O.J. (2013). Bioremediation of soil contaminated by diesel oil using Pleurotus pulmonarius Fries (Quelet) and its effects on the growth of Corchorus olitorius (L.). Journal of Applied Biosciences, 68: 5366 – 5373.
Adenipekun, C.O. and Fasidi, I.O. (2005). Degradation of selected agricultural wastes by Pleurotus tuber-regium (Fries) Singer and Lentinus subnudus (Berk) – Nigerian Edible Mushrooms. Advances in Food Science, 27(2): 61–64.
Adenipekun, C.O. and Isikhuemhen, O.S. (2008). Bioremediation of Engine Oil Polluted Soil by the Tropical White Rot Fungus,Lentinus squarrosulus Mont. (Singer). Pakistan Journal of Biological Sciences, 11: 1634-1637.
Adenipekun, C.O. and Kassim, L.Q. (2006). Effects of spent engine oil on some growth parameters and moisture content of Celosia argentea L. Nigerian Journal of Botany, 19(2):318-324.
Adenipekun C.O., Olanrewaju, O.O. and Ogunjobi, A. A. (2011). Bioaccumulation of Heavy metals and Nutrient content Supplementation by two White rot fungi in Crude oil polluted soils. Researcher, 3(5):13-20.
Adenipekun, C.O. and Omoruyi, O.M. (2008). Bioremediation of contaminated soils by Pleurotus ostreatus (Jacq. Fr.) P. Kumm. Nigerian Journal of Botany, 21(2): 274–279..
Adesuyi, A.A., Nnodu, V.C., Njoku, K.L. and Jolaoso, A. (2015). Nitrate and Phosphate Pollution in Surface Water of Nwaja Creek, Port Harcourt, Niger Delta, Nigeria. International Journal of Geology, Agriculture and Environmental Sciences, 3(5), 14-20.
Agarry, S.E., Olu-Arotiowa, O.A., Aremu, M.O. and Jimoda, L.A. (2013). Biodegradation of Dichlorovos (Organophosphate Pesticide) in Soil by Bacterial Isolates. Journal of Natural Sciences Research, 3(8): 12-16.
Ahmed, M., Ismail, S. and Mabrouk, S. (1998). Residues of some chlorinated hydrocarbon pesticides in rain water, soil and ground water, and their influence on some soil microorganisms. Environmental International, 24(5-6): 665-670.
Alexander, M. (1999). Biodegradation and Bioremediation. 2nd Edition. Academic Press: New York, NY.
Asogwa, E.U. and Dongo, L.N. (2009). Problems associated with pesticide usage and application in Nigerian cocoa production: A review. African Journal Agricultural Research, 4(8): 675-683.
Atagana, H., Haynes, R. and Wallis, F. (2003). The use of surfactants as possible enhancers in bioremediation of creosote contaminated soil. Water, Air and Soil Pollution; 142: 137-149.
ATSDR (1997). Toxicological profile for dichlorvos. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. Agency for Toxic Substances and Disease Registry (ATSDR), pp. 99-110.
ATSDR (2005). Toxicological profile for Hexachlorocyclohexane. Atlanta, GA: U.S. Department of Health and Human Services, Public Health Service. Agency for Toxic Substances and Disease Registry (ATSDR),
Aust, S.D. and Swanner, P.R. (2003). Detoxification and Metabolism of chemicals by white-rot fungi .In: Pesticide decontamination and detoxification. J.J.P.C., Zhu. S.D. Aust. T Lemley Gan, 3-14. Washington, D.C. Oxford University Press.
Bates, R.A. (1954). Electrometric determination. John Wiley Sons, Inc. New York.
Bending, G.D., Friloux, M. and Walker, A. (2002). Degradation of contrasting pesticides by white rot fungi and its relationship with ligninolytic potential. FEMS Microbiology Letters, 18:212(1):59 – 63.
Chedi, B. A. Z. and Aliyu, M. (2010). Effect and Management of Acute Dichlorvos Poisoning in Wistar Rats. Bayero Journal of Pure and Applied Sciences, 3(2): 1 – 3.
Cork, D.J. and Krueger, J.P. (1991). Microbial transformation of herbicides and pesticides. Advances in Applied Microbiology, 36: 1-66.
Dua, M.,Singh, A., Sethunathan, N. and Johri, A.K. (2002). Biotechnology and bioremediation: successes and limitations. Applied Microbiology and Biotechnology, 59(2-3): 143-52. 
Fragoeiro, S. I. S. (2005). Use of Fungi in Bioremediation of Pesticides. Ph.D Dissertation, Cranfield University.
 
Furukawa, K. (2003). Super bugs for Bioremediation. Trends in Biotechnology, 21: 187- 190.
Gruter, A., Friederich, U. and Wurgler, F.E. (1991). The mutagenicity of edible mushrooms in a histidine-independent bacterial test system. Food Chemistry, 4:159–165.
Haritash, A.K. and Kaushik, C.P. (2009) Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): A review. Journal of Hazard Mater, 169:1–15.
Harris, J. and Steer, J. (2003). Modern methods for estimating soil microbial mass and diversity: and integrated approach. In Sasek V. et al. (eds.) The utilization of bioremediation to reduce soil contamination: Problems and solutions, pp. 29-48. Kluwer Academis Publishers.
Hernanadez, F. Sancho, J. and Pozo, O. (2005). Critical review of the application of liquid chromatography/mass spectrometry to the determination of pesticide residues in biological samples. Analytical and Bioanalytical Chemistry 382(4):934-946.
Hofrichter, M., Vare, T., Kalsi, M., Galkin, S., Scheibner, K., Fritsche, W. and Hatakka, A. (1999). Production manganese peroxidase and organic acids and mineralization of 14C-labelled lignin (14C-DHP) during solid-state fermentation of wheat straw with the white ofrot fungus Nematoloma frowardii. Applied Environmental Microbiology, 65: 1864-1870.
Hossain, M.S. and Anantharaman, N. (2006). Activity Enhancement of Ligninolytic Enzyme of Trametes versicolor with Bagasse Powder. African Journal of Biotechnology, 5:189-194.
Kearney, P. and Wauchope, R. (1998). Disposal options based on properties of pesticides in soil and water. In: Kearney P. and Roberts T. (Eds.) Pesticide remediation in soils and water. Wiley Series in Agrochemicals and Plant Protection.
Kulshreshtha, S., Mathur, N. and Bhatnagar, P. (2014). Mushroom as a product and their role in Mycoremediation. AMB Express, 4:29. doi:  10.1186/s13568-014-0029-8
Kulshreshtha, S., Mathur, N. and Bhatnagar, P. (2013). Cultivation of Pleurotus citrinopileatus on handmade paper and cardboard industrial wastes. Industrial Crop Production, 2013;4:340–346.
Kuo, W. and Regan, R. (1998) Aerobic carbamate bioremediation aided by compost residuals from the mushroom industry: laboratory studies. Compost Science & Utilization; 6 (1): 19-29.
La Dreau, Y., Jacquot, F., Doumena, P., Guilliano, M., Betrand, J. and Mille, G.(1997). Hydrocarbon balance of a site which had been highly and chronically contaminated by petroleum waste of a refinery from 1956-1992.Marine Pollution Bulletin 34: 456-468.
Marin, S., Sanchis, V., Ramos, A. and Morgan, N. (1998). Effect of water activity on hydrolytic enzyme production by Fusarium moniliforme and Fusarium proliferatum during colonization of maize. International Journal of Food Microbiology, 42(3):185 – 194.
Nawaz, K., Hussain, K., Choudary, N., Majeed, A., Ilyas, U., Ghani, A., Lin, F., Ali, K., Afgan, S., Ghulam, R. and Lasari, M.I. (2011). Eco-friendly role of biodegradation against agricultural pesticides hazards. African Journal of Microbiology Research, 5(3): 177-183.
Nerud, F., Baldrian, J., Gabriel, J. and Ogbeifun, D. (2003). Nonenzymic degradation and decolorization of recalcitrant compounds. In Sasek V. et al. (Eds.) The utilization of bioremediation to reduce soil contamination: Problems and solutions, pp. 29-48. Kluwer Academis Publishers.
Njoku, K.L., Yussuf, A., Akinola, M.O., Adesuyi, A.A., Jolaoso, A.O. and Adedokun, A.H. (2016). Mycoremediation of Petroleum hydrocarbon polluted soil by Pleurotus pulmonarius. Ethiopia Journal Environmental Sciences and Management, 9(Suppl. 1): 865 – 875.
Njoku, K.L., Ezeh, C.V., Obidi, F.O. and Akinola, M.O. (2017). Assessment of Pesticide Residue Levels in Vegetables sold in some Markets in Lagos State, Nigeria. Nigerian Journal of Biotechnology, 32: 53-60.
Novotny, C., Erbanova, P., Sasek, V., Kubatova, A., Cajthman, T., Lang, E., Krahl, J. and Zadrazil, F. (1999). Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi. Biodegradation; 10: 159-168.
Oei, P. (1996). Mushroom cultivation with special emphasis on appropriate techniques for developing countries.Tool Publications, Leiden, The Netherlands, pp 94-119.
Okeke, B., Smith, J., Paterson, A. and Watson-Craik, I. (1996). Influence of environmental parameters on pentachlorophenol biotransformation in soil by Lentinula edodes and Phanerochaete chrysosporium. Applied Microbiology and Biotechnology, 45:263-266.
Okparanma, R.N., Ayotamuno, J.M., Davis, D.D. and Allagoa, M. (2011). Mycoremediation of polycyclic aromatic hydrocarbons (PAH) - contaminated oil-based drill-cuttings. African Journal of Biotechnology, 10(26):5149-5156.
Osano, A.A., Siboe, G.M., Ochanda, J.O. and Kokaro. J.O. (1999). Biodegradation of DDT: The Role of Pleurotus sp., A Lingnicolous Fungus. Bioremediation of Nitroaromatic and Haloaromatic Compounds. B.C. Alleman and A. Leeson, Eds. Columbus, OH: Battelle Press.
Philippoussis, A., Diamantopoulou, P., Euthimiadou, H. and Zervakis, G. (2011). The composition and porosity of lignocellulosic substrates influence mycelium growth and respiration rates of Lentinus edodes. International Journal of Medicinal Mushroom, 3(2-3): 198.
Quimio, T.H., Chang, S.T. and Royse, D.J. (1990). Technical guidelines for mushrooms growing in the Tropic. FAO Plant Production and Protection, No. 106, Rome. pp152.
Reddy, C. and Mathew, Z. (2001). Bioremediation potential of white rot fungi. In. Gadd G. (Eds.) Fungi in bioremediation. Cambridge University Press. Cambridge, U.K.
Rigas, F., Papadopoulou, K., Dritsa, V. and Doulia, D. (2007). Bioremediation of a soil contaminated by lindane utilizing the fungus Ganoderma australe via response surface methodology. Journal of Hazardous Materials, 140: 325–332.
Safferman, S.I., Lamar, R.T., Vonderhaar, S., Neogy, R., Haught, R.C. and Krishnan, E.R. (1995). Treatability study using Phanerochaete sordida for the Bioremediation of DDT Contaminated Soil. Toxicological and Environmental Chemistry, 50: 237-251
Sasek, V. (2003). Why mycoremediations have not yet come to practice. In : Sasek V. et al. (Eds.) In: The utilization of bioremediation to reduce soil contamination: Problems and solutions, pp. 247-276. Kluwer Academis Publishers, 2003.
Smalling, K.L. and Kuivila, K.M. (2008). Multi-residue method for the analysis of 85 current use and lecacy pesticides in bed and suspended sediments. Journal of Chromatography A, (1)8-18.
Surekha, R.M., Lakshmi, P.K.L., Suvarnalatha, D., Jaya, M., Aruna, S., Jyothi, K., Narasimha, G. and Venkateswarlu, K. (2008). Isolation and characterization of a chlorpyrifos degrading bacterium from agricultural soil and its growth response. African Journal of Microbiological Research, 2: 26-31.
Udochukwu, U., Nekpen, B.O., Udinyiwe, O.C. and Omeje, F.I. (2014). Bioaccumulation of Heavy metals and pollutants by edible mushroom collected from Iselu market Benin-city. Internationaal Journal of Current Microbiology and Applied Sciences, 3(10): 52-57
USDA (2011). Soil Quality Kit – Guides for Educators. Retrieved online from: https://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs142p2_053293.pdf. Accessed on 30th December, 2016.
Veignie, E., Rafin, C., Woisel, P. and Cazier, F. (2004). Preliminary evidence of the role of hydrogen peroxide in the degradation of benzo[a]pyrene by a non-white rot fungus Fusarium solani. Environmental Pollution, 129: 1-4.
Wackett, L.P. and Hershberger, C.D. (2001). Biocatalysis and Biodegradation: Micrbial Transformation  of Organic Compounds. ASM Press, Washington DC. 228pp.
Zak, D., Holmes, W., MacDonald, N. and Pregitzer., K. (1999). Soil temperature, matric potential, and the kinetics of microbial respiration and nitrogen mineralization. Soil Science Society of America Journal, 63: 575-584.
Pollution
Volume 4, Issue 4
October 2018
Pages 605-615

Files

Share

How to cite

Statistics