Nitrate Bioremoval by Phytotechnology using Utricularia aurea Collected from Eutrophic Lake of Theerthamkara, Kerala, India

Document Type : Original Research Paper

Authors

1 Department of Environmental Science, Division of Environmental Microbiology, PSG College of Arts and Science, TN-641014, India Department of Environmental Science, Central University of Kerala, KL- 671320, India

2 Department of Environmental Science, Central University of Kerala, KL- 671320, India

Abstract

The aim of this study was to compare the selected aquatic plants ability to remove nitrate from wastewater. Excess of these nutrients in water can directly affect human health (methemoglobinaemia) or indirectly through the products of secondary pollution include eutrophication. Negative impact of nutrients excess in surface water often causes the destruction of water ecosystems, and therefore, common substances of these elements must be monitored and managed. Spectrophotometric technique was commonly used for quick and simple analyses of nutrients in waste water. There are calibration curves for each nutrient and for the determination of their concentration. Phytotechnology is one of the biological wastewater treatment methods or processes to eliminate nitrate contaminant from aquatic system. So as to avoid the eutrophic formation of fresh water and to determine the efficiency of nitrate utilization by specific aquatic plants which include Utricularia aurea and Salvinia molesta were collected from a eutrophic lake at Theerthamkara, Kerala. The samples were allowed to grow in nitrate solution for about one month at different concentrations. The optical density (OD) of nitrate solution at 410 nm was measured on alternative days of the experiment by using UV spectrophotometer. After 33 days of treatment periods, the maximum amount of nitrate removed in terms of percentage was found to be 95% by Utricularia aurea and 92% by Salvinia molesta at 100 ppm nitrate concentration. The results revealed that the aquatic plant (carnivorous) based system of phytotechnology was productively removed the nitrate load from the synthetic wastewater containing nitrate.

Keywords


Abdel-Raouf, N., Al-Homaidan, A.A. and Ibraheem, I.B.M., (2012). Microalgae and wastewater treatment. Saudi J. Biol. Sci., 19:257–275. doi:10. 1016/j.sjbs.2012.04.005.
Amalina Amirah Bt Abu Bakar, Khairul Ammar B., Muhd Ali, Nurul Athirah Bt Ahmad Tarmizi,, Ahmad Zia Ul-Saufie B., Mohamad Japeri. and Nor Janna Bt Tammy., (2016). Potential of using bladderwort as a biosorbent to remove zinc in wastewater. International Conference on Advanced Science, Engineering and Technology (ICASET) 2015 AIP Conf. Proc. Vol:774(1), 030023-1–030023-6; doi: 10.1063/1.4965079 Published by AIP Publishing. 978-0-7354-1432-7/$30.00
APHA. (1998). American Public Health Association, American Water Works Association, Water Environment Federation. Standard Methods for the Examination of Water and Wastewater.
Barber ,W.P. and Stuckey, D.C., (2000). Nitrogen removal in a modified anaerobic baffled reactor (ABR): 1, Denitrification. Water Res., 34: 2413–2422.
Chiban, M., Soudani, A., Sinan, F. (2012). Removal of nitrate ions by using low-cost adsorbents: Equilibrium isotherm, kinetics and thermodynamic study. Nova Science Publishers. 31-48.
Dejam, A., Hunter, C.J., Pelletier, M.M., Hsu, L.L., Machado, R.F., Shiva, S., Power, G.G., Kelm, M., Gladwin, M.T., Schechter, A.N. (2005). Erythrocytes are the major intravascular storage sites of nitrite in human blood. Blood, 106: 734 -739.
Health Canada. Canadian Perinatal Health Report. (2003). Ottawa: Minister of Public Works and Government Services Canada.
Joanna Augustynowicz, Krzysztof Łukowicz, Krzysztof Tokarz, Bartosz Jan Płachno. (2015). Potential for chromium (VI) bioremediation by the aquatic carnivorous plant Utricularia gibba L. (Lentibulariaceae), Environ. Sci .Pollut. Res., 22:9742–9748. DOI 10.1007/s11356-015-4151-1.
Jorgensen, S.E. (2001). Water quality: The impact of eutrophication. UN Environment Programme.
Keisham Bijyalakshmi Devi, Dharitri Borah, Jayashree Rout. (2018). Algal colonization on an insectivorous plant, Utricularia aurea Lour. in a freshwater marsh of southern Assam, India Epiphytic algae on Utricularia aurea. Phykos 48 (2): 17-31.
Mikuska, P. and Vecera, Z. (2003). Simultaneous Determination of Nitrite and Nitrate in Water by Chemiluminescent Flow-injection Analysis. Analytica Chimica Acta., 495(1-2): 225-232.
Mwagona, P.C., Yao, Y., Yuanqi, S. and Yu, H. (2019). Laboratory study on nitrate removal and nitrous oxide emission in intact soil columns collected from nitrogenous loaded riparian wetland, Northeast China. PLoS ONE 14(3): e0214456.
https://doi.org/10.1371/journal.pone.0214456.
Nor Baizura Bt Hamid, Masiri B, Kaamin, Mardiha Bt Mokhtar, Aslila Bt Abd Kadir,  Siti Khatijah Bt Abu Bakar, Nurul Huda Bt Ibrahim. (2015). Research on the Effectiveness of an Aquatic Plant (Utricularia aurea) for Fish Preservation., J. Civil Engg. Res., 5:1-5.
Ozturk, N. and  Bektas, T.E .(2004). Nitrate removal from aqueous solution by adsorption onto various materials. J. Hazard. Mater., 112: 155-162.
Rawat SK, Singh RK, Rana P. Singh (2012) Remediation of nitrite contamination in ground and surface waters using aquatic macrophytes. J. Environ. Biol., 33: 51-56.
Saura, R., Silva, Robert Gibson, Lubomír Adamecd, Yoannis Domíngueze, Vitor Miranda. (2018). Molecular phylogeny of bladderworts: A wide approach of Utricularia (Lentibulariaceae) species relationships based on six plastidial and nuclearDNA sequences. Mol. Phylogene. Evolu., 118: 244–264.
Seidel, C., Gorman, C. and Werner, K. (2008). Nitrate reduction by sulphur modified iron: pilot study results. Presentation at the American Water Works Association Inorganic Contaminants Workshop.
Taylor, P. (1989). Genus Utricularia: A Taxonomic Monograph, first ed. Royal Botanic Gardens, Kew, London. New Taxa
U.S. EPA. (U.S. Environmental Protection Agency). (1987). Nitrate/Nitrite: Health Advisory. Washington, DC: Office of Drinking Water.
Usharani, K., Muthuchamy, M. and Lakshmanaperumalsamy, P. (2011). Biological removal of phosphate from synthetic wastewater using bacterial consortium, Iran. J. Biotechnol., 9(1): 37-49.
Usharani, K., Sruthilaya, K. and Divya, K. (2017). Determination of nitrate utilization efficiency of selective strain of Bacillus sp. isolated from Eutrophic Lake, Theerthamkara, Kasaragod, Kerala. J. Poll., 3(1): 55-67.
Usharani, K. and Keerhi, K.V. (2018). Nitrate removal by plant based bioremediation using Utricularia aurea from eutrophic Lake of Theerthamkara, India. Conference Proceedings and E-Book of Abstracts of the Joint Conference 7th European Bioremediation Confrence & 11th International Society of Environmental Biotechnology Conference-2018; Chania, Crete, Greece, June 25-28, 2018, ISBN 978-618-81537-6-9.pp 103-104.
Viessman, Jr. W. and Hammer, M.J. (2005). Water supply and pollution control, seventh ed., Pearson Prentice Hall, Upper Saddle River, NJ.
WHO. (1984). Drinking Water Quality Control in Small Community Supplies. In : Guidelines for Drinking Water Quality (vol 3), WHO, Geneva, Switzerland.
WHO. (2007). Nitrate and nitrite in drinking-water. Background document for development of WHO Guidelines for Drinking-water Quality. World Health Organization, Geneva (WHO/SDE/WSH/07.01/16).