Biomonitoring of Heavy Metals Accumulation with Pseudevernia furfuracea (L.) Zopf in Kırşehir Province, Türkiye

Document Type : Original Research Paper

Authors

Ankara University, Faculty of Science, Department of Biology, TR-06100, Tandoğan, Ankara, Türkiye

Abstract

Today, the problem of air pollution has been highlighted by rapid population growth and urbanisation, along with the development of industry. Over the last fifty years, much attention has been paid to the relationship between lichens and airborne particulate matter (especially heavy metals). The use of living organisms in air pollution studies is now widely accepted in many countries and the results of these biomonitoring studies are very important for future action. The goal of this study was to determine heavy metals in Kırşehir province using the bag technique, a biomonitoring approach, with Pseudevernia furfuracea (L.) Zopf lichen and to develop a pollution map of the city. In November 2002, lichen specimens were obtained from an unpolluted region in the Yapraklı Mountains, Çankırı, and transplanted to 4 distinct places in Kırşehir. After 3 and 6 months of exposure, they were collected in order to analyse heavy metals (Cu, Cd, Mn, Ni, Pb and Zn) with Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). In addition, chlorophyll-a and chlorophyll-b contents were determined by Dimethyl sulfoxide (DMSO) method. The findings revealed that the heavy metal contents in various stations are the result of industrial, traffic, and heating activities. As a result, P. furfuracea showed excellent bioindicator ability for detecting air pollution.

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Main Subjects


Abas, A., Aiyub, K., & Awang, A. (2022). Biomonitoring Potentially Toxic Elements (PTEs) Using Lichen Transplant Usnea misaminensis: A Case Study from Malaysia. Sustainability 2022, 14, 7254. https://doi.org/10.3390/su14127254
Aguilera, A., Bautista, F., Gutiérrez-Ruiz, M., Ceniceros-Gómez, A.E., Cejudo, R., & Goguitchaichvili, A. (2021). Heavy metal pollution of street dust in the largest city of Mexico, sources and health risk assessment. Environ. Monit. Assess. 16;193(4):193.
Akkan, T., Yazıcıoğlu, O., Yazıcı, R., & Yılmaz, M. (2018). An examination of ecological and statistical risk assessment of toxic metals in sediments at Sıddıklı Dam Lake: A Case Study in Kırşehir, Turkey. Fresenius environmental bulletin 27(12):8104-8111.
Baddeley, M.A., Ferry, B. W., & Fınegan, E. J. (1972). The effects of sulphur dioxide on lichen respiration, Lichenologist, 5, 283-291.
Barnes J, D., Balaguer, L., Manrique, E., Elvira, S., & Davison, A, W. (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environ. Exp. Bot. 32, 85-100. 
Bermudez, G.M.A., Rodriguez, J.H., & Pignata, M.L. (2009). Comparison of the air pollution biomonitoring ability of three Tillandsia species and the lichen Ramalina celastri in Argentina,2009,Environmental Research,Volume 109, Issue 1,2009,Pages 6-14,ISSN 0013-9351, https://doi.org/10.1016/j.envres.2008.08.014.
Boamponsem, L.K., De Freitas, C.R., & Williams, D. (2017). Source apportionment of air pollutants in the Greater Auckland Region of New Zealand using receptor models and elemental levels in the lichen, Parmotrema reticulatum. Atmos. Pollut. Res. 8,101–113. https://doi.org/10.1016/j.apr.2016.07.012.
Boonpeng, C., Sangiamdee, D., Noikrad, S., & Boonpragob, K. (2023).Lichen biomonitoring of seasonal outdoor air quality at schools in an industrial city in Thailand. Environ Sci Pollut Res Int. 2023 May;30(21):59909-59924. doi: 10.1007/s11356-023-26685-z. 
Brodo, I M. (1961). Transplant experiments with corticolous lichens using a new technique. Ecology. 42, 838–841.
Brodo, I. M., Sharnoff, S. D., & Sharnoff, S. (2001). Lichens of North America. New Haven: Yale University Press.
Chahloul, N., Khadhri, A., Vannini, A., Mendili, M., Raies, A., & Loppi, S. (2023). Selecting the species to be used in lichen transplant surveys of air pollution in Tunisia. Environ Monit Assess. 2023 Apr 14;195(5):570. doi: 10.1007/s10661-023-11219-4. 
Chettri, M.K., Cook,C.M., Vardaka, E., Sawidis,T., & Lanaras, T. (1998). The effect of Cu, Zn and Pb on the chlorophyll content of the lichens Cladonia convoluta and Cladonia rangiformis, Environmental and Experimental Botany,Volume 39, Issue 1,1998,Pages 1-10,ISSN 0098-8472,https://doi.org/10.1016/S0098-8472(97)00024-5.
Çiftçi, H., Er, Çalışkan Ç., Aslanhan, E., & Aktok, E. (2021). Monitoring of heavy metal pollution by using populus nigra and cedrus libani. Sigma J Eng Nat Sci 2021;39(4):367–373.
Conti, M.E., & Cecchetti, G. (2001). Biological monitoring: lichens as bioindicators of air pollution assessment: a review. Environ. Pollut. 114, 471–492.
DIE (Turkısh Statistical Instıtute) 2002. Available at http://www.die.gov.tr/nufus/02012002T3
Frati, L., & Brunialti, G.(2023). Recent Trends and Future Challenges for Lichen Biomonitoring in Forests. Forests 2023, 14, 647. https://doi.org/10.3390/f14030647
Gao, G., Zeng, H., & Zhou, Q. (2022). Biomonitoring Atmospheric Pollution of Polycyclic Aromatic Hydrocarbons Using Mosses. Atmosphere 2022, 14, 26. https://doi.org/10.3390/atmos14010026
Garty, J. (2001). Biomonitoring atmospheric heavy metals with lichens: Theory and Application. Crit. Rev. Plant Sci. 2001, 20, 309–371.
Gonzalez, C. M., & Pignata, M. L. (1994). The influence of air pollution on soluble proteins, chlorophyll degradation, MDA, sulphur and heavy metals in a transplanted lichen. Chem. Ecol. 9, 105–113.
Halıcı, M., Odabaşoğlu, F., Aslan, A., Çakır, A, Süleyman, H., Karagöz, Y., & Bayır, Y. (2005). Antioxidant activity, reducing power and total phenolic content of some lichen species. Fitoterapia. 76 (2005) 216 – 219.
Işık, V., Vardar, Çiğdem, Aksoy, A., & Yıldız, A. (2023). Biomonitoring of heavy metals by Pseudevernia furfuracea (L.) Zopf in Aksaray city, Turkey. EQA - International Journal of Environmental Quality, 56(1), 52–61. https://doi.org/10.6092/issn.2281-4485/16426
Kauppi, M. (1980), Fluorescence microscopy and microfluorometry fort he examination of pollution damage in lichens, Annales Botanici Fennici 17, 163-173.
Loppi, S., Ravera, S., & Paoli, L. (2019) Coping with uncertainty in the assessment of atmospheric pollution with lichen transplants, Environmental Forensics, 20:3, 228-233, DOI: 10.1080/15275922.2019.1627615
Lucadamo, L., Gallo, L., &  Corapi, A. (2022).  Detection of air quality improvement within a suburban district (southern Italy) by means of lichen biomonitoring . Atmospheric Pollution Research. 13(3), 101346. https://doi.org/10.1016/j.apr.2022.101346.
Martínez-Guijarro, R.,  Paches , M.,  Romero, I., & Aguado, D.(2021). Sources, Mobility, Reactivity, and Remediation of Heavy Metal(loid) Pollution: A Review. Adv Environ Eng Res 2021; 2(4), doi:10.21926/aeer.2104033.
Niewiadomska, E., Jarowiecka,D., & Czarnota,P. (1998). Effect of different levels of air pollution on photosynthetic activity of some lichens. Acta Societatis Botanicorum Poloniae.  Vol 67, No 3-4 (1998).
Palabıyık E, Yılmaz M, Çalışkan ÇE, Çiftçi H, & Palabıyık AA. (2022). The Accumulation of some heavy metals in northern pike (Esox lucius L., 1758) inhabiting Sıdıklı Küçükboğaz Dam Lake (KIRŞEHİR) LimnoFish. 8(3): 243-250. doi: 10.17216/LimnoFish.1024588.
Puckett, K. J., Nıeboer, E., Flora, W. P.,  & Rıchardson, D . H. S. (1973). Sulphur dioxide: its effects on photosynthetic *^C fixation in lichens and suggested mechanisms of phytotoxicity. New Phytologist, 72,141-154.
Richardson, D. H. S.,  & Puckett, K. J. (1973). Sulphur dioxide and photosynthesis in lichens. In: Air Pollution and Lichens (Ed. by B. W. Ferry, M. S. Baddeley & D. L. Hawksworth), pp. 283-298. The Athlone Press, University of London.
Ronen, R., Canaanı, O., Garty, J., Cahen, D., Malkın, S., & Galun, M. (1984). The effect on air pollution and bisulfite treatment in the lichen Ramalina duriaei studied by photoacoustics. In: Advances in Photosynthesis Research. Proceedings of the Sixth International Congress on Photosynthesis, August 1-6 1983, Brussels.
Ramić, E., Huremović, J., Muhić-Šarac, T., Dug, S., Zero, S., & Olovcic, A. (2019). Biomonitoring of Air Pollution in Bosnia and Herzegovina Using Epiphytic Lichen Hypogymnia physodes. Bull Environ Contam Toxicol 102, 763–769 (2019). https://doi.org/10.1007/s00128-019-02595-0
Riddell, J., Nash, III T. H., & Padgett, P. (2008). The effect of HNO3 gas on the lichen Ramalina menziesii. Flora. 203, 47-54.
Saib, H., Yekkour, A., Toumi, M., Guedioura, B., Benamar, M.A., Zeghdaoui, A., Austruy, A., Bergé-Lefranc, D.,  Culcasi, M., & Pietri, S. (2023). Lichen biomonitoring of airborne trace elements in the industrial-urbanized area of eastern algiers (Algeria),Atmospheric Pollution Research,Volume 14, Issue 1,2023,101643,ISSN 1309-1042, https://doi.org/10.1016/j.apr.2022.101643.
Scerbo, R., Ristori, T., Possentin, L., Lampugnani, L., Barale, R., & Barghigiani, C. (2002). Lichen (Xanthoria parietina) biomonitoring of trace element contamination and air quality assessment in Pisa Province (Tuscany, Italy). Sci. Total Environ. 286, 27–40. https://doi.org/10.1016/s0048-9697(01)00959-7.
Sczepaniak, K., & Biziuk, M. (2003). Aspects of the biomonitoring studies using mosses and lichens as indicators of metal pollution. Environ. Res. 93, 221–230.
Showman, R. E. (1972). Residual effects of sulfur dioxide on the net photosynthetic and respiratory rates of lichen thalli and cultured lichen symbionts. Bryologist, 75, 335-341
Sujetoviene, G. (2015). Monitoring lichen as indicators of atmospheric quality. In Recent Advances in Lichenology, Springer: New Delhi, India, 2015; pp. 87–118.
Syed Salleh, S.N.A., & Abas, A. (2023). Monitoring Heavy Metal Concentrations Using Transplanted Lichen in a Tourism City of Malaysia. Sustainability 2023, 15, 5885. https://doi.org/10.3390/su15075885.
Yıldız, A., Aksoy, A., Tug, G.N., & Işlek, C. (2008). Biomonitoring of heavy metals by Pseudevernia furfuracea (L.) Zopf in Ankara (Turkey). J Atmos Chem 60, 71–81 (2008). https://doi.org/10.1007/s10874-008-9109-y 
Yıldız, A., Aksoy, A., Akbulut, G., Demirezen, D., İşlek, C., Altuner, E., & Duman, F, (2011) Correlation between chlorophyll degradation and the amount of heavy metals found in Pseudevernia furfuracea in Kayseri ( Ekoloji 20 78 82 88 https ://doi org/10 5053 /ekoloji 2011 7813.
Yıldız, A., Vardar, Ç., Aksoy, A., & Ünal, E, (2018). Biomonıtoring of heavy metals deposition with Pseudevernia furfuracea (L Zopf in Çorum city Turkey Journal of Scientific Perspectives. https :://doi org/ 10 26900 /jsp 2018 02.
Web. Retrieved September,15 2023, from https://www.dogakolik.com/cankiri/yaprakli-buyuk-yayla/.
Wolterbeek, B. (2002). Biomonitoring of trace element air pollution: principles, possibilities and perspectives. Environ. Pollut. 120, 11–21.