Comparing the Effect of Kerosene Pollution on Forest and Industrial Soil Microbial Community

Document Type : Original Research Paper

Authors

1 Department of Microbiology, Sirja branch, Islamic Azad University, Kerman, Iran

2 Department of Microbiology, Sirjan Branch, Islamic Azad University, Sirjan, Iran

3 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

10.7508/pj.2016.03.010

Abstract

Kerosene is the colorless liquid and slightly heavier than gasoline that
specific odor removes after evaporation. Soil and underground water source are
contaminated with different pollutants such as petroleum hydrocarbons. These pollutants
have various negative environmental effects on soil and surrounding environment. The
aim of this research is to understand the effect of kerosene pollution on two different
soils. The two different collected soils include Industrial and Forest soil. Six microcosms
were designed. Indeed, each soil has three microcosms: unpolluted microcosm, polluted
microcosm, and polluted microcosm with nutrient (Nitrogen and Phosphor). Some factors
were assayed in each microcosm during 120 day of experiment. These factors include
total heterotrophic bacteria, total kerosene degrading bacteria, dehydrogenase enzyme,
and kerosene biodegradation. The results of this study show that the highest quantity of
heterotrophic bacteria is related to forest soil (6×109). The quantities of kerosene
degrading bacteria significantly were lower than heterotrophic bacteria in all soil
microcosms. The quantity of kerosene degrading bacteria have decrement pattern until
60th day of experiment, but, after this day, these bacteria have increment pattern. The best
dehydrogenase activity between different microcosms is related to polluted microcosm
with kerosene except for farmland soil. The highest biodegradation of kerosene in all
studied soil belongs to industrial microcosm (95%). Statistical analysis of the results
shows that there is a significant correlation between MPN quantity of heterotrophic
bacteria and other assayed factrs. Also, forest soil has significant difference with other
soils. It may be possible to propose appropriate strategies for bioremediation of different
studied soil types using the results obtained in this research.

Keywords

References

Alef, K. and Nanniper, P. (1995). Methods in applied soil microbiology and biochemistry, (Academic Press: New York). 228.
Amadi, A., Samuel, D. and Anthony, N. (1996). Chronic effects of oil spill on soil properties and microflora of a rainforest ecosystem in Nigeria. Water, Air, and Soil Pollut., 86, 1-11.
Barathi, S. and Vasudevan, N. (2001). Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from petroleum contaminated soil. Environ. Int., 26, 413-416.
Bayat, Z., Hassanshahian, M., Askeri Hesni, M. (2016). Study the symbiotic crude oil-degrading bacteria in the mussel Mactra stultorum collected from the Persian Gulf. Marine Pollution Bulletin. 105 (1), 120–124.
Cappello, S., Denaro, R., Genovese, M., Giuliano, L. and Yakimov, M.M. (2006). Predominant growth of Alcanivorax during experiments on oil spill bioremediation in mesocosms. Microbiol. Res., 162, 185-190.
Del Arco, J.P. and De Franca, F.P. (2001). Influence of oil contamination levels on hydrocarbon biodegradation in sandy sediment. Environ.Pollut., 110, 515-519.
Delille, D. and Coulon, F. (2008). Comparative mesocosm study of biostimulation efficiency in two different oil-amended sub-Antarctic soils. Microb. Ecol., 56, 243-252.
Emtiazi, G., Saleh, T. (2009). Hassanshahian, M. The effect of bacterial glutathione S-transferase on morpholine Degradation. Biotechnol. J. , 4, 202–205.
Hassanshahian, M., Emtiazi, G., Cappello, S. (2012a). Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar. Pollut. Bull. 64, 7–12.
Hassanshahian, M., Tebyanian, H., Cappello, S. (2012b). Isolation and characterization of two crude-oil degrading yeast strains, Yarrowia lipolytica PG-20 and PG-32 from Persian Gulf. Mar. Pollut. Bull. 64, 1386–1391.
Hassanshahian, M., Ahmadinejad, M., Tebyanian, H., Kariminik, A. (2013). Isolation and characterization of alkane degrading bacteria from petroleum reservoir waste water in Iran (Kerman and Tehran provenances). Mar. Pollut. Bull. 73, 300–305.
Ives, A.R., Foufopoulos, J., Klopfer, E.D., Klug, J.L. and Palmer, T.M. (1996). Bottle or big-scale studies: how do we do ecology. Ecol., 77, 681-685.
Kasai, Y., Kishira, H., Sasaki, T., Syutsubo, K., Watanabe, K. and Harayama, S. (2002). Predominant growth of Alcanivorax strains in oil-contaminated and nutrient supplemented sea water. Environ. Microbio., 4, 141-147.
Lee, M., Kim, M.K., Singleton, I., Goodfellow, M. and Lee, S.T. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. J. Appl. Microbiol., 100, 325–333.
Li, Z.Y., Kravchenko, I., Xu, H. and Zhang, C. (2007). Dynamic changes in microbial activity and community structure during biodegradation of petroleum compounds: A laboratory experiment. J. Environ. Sci., 19, 1003–1013.
Nisha, P., Nayana, M. and Varghese, M. (2013). Degradation Studies on Diesel Oil Using Bacterial Consortium Isolated From Oil Polluted Soil. Adv. Biotechnol. 13(2), 06-14.
Mukherji, S. and Vijay, A. (2002). Critical issues in bioremediation of oil and tar contaminated sites. In: Proceedings of the International Conference on Advances in Civil Engineering, Civil Eng. Dept., IIT Kharagpur, India, 3–5 January 2002, pp. 507-516.
Ojimba, T. (2012). Determining the effects of crude oil pollution on crop production using stochastic trans log production function in Rivers State, Nigeria. J. Develop. Agri. Eco., 4(13), 346-360.
Radwan, S.S., Al-Hasan, R.H., Salamah, A. and Khanafer, M. (2005). Oil-consuming microbial consortia floating in the Arabian Gulf. Int. Biodeterio. Biodeg., 56, 28-33.
Rahman, K.S.M., Thahira-Rahman, J., Lakshmanaperumalsamy, P. and Banat, I.M. (2004).Towards efficient crude oil degradation by a mixed bacterial consortium. Biores.Technol., 85, 257-261.
Riffaldi, R., Levi-minzi, R., Cardelli, R., Palumbo, S. and Saviozzi, A. (2006). Soil biological activities in monitoring the bioremediation of diesel oil-contaminated soil. Water, Air and Soil Pollut., 170, 3-15.
Robertson, S., Rutherford, P.M. and Massicotte, H.B. (2011). Plant and soil properties determine microbial community structure of shared Pinus-Vaccinium rhizospheres in petroleum hydrocarbon contaminated forest soil. Plant. Soil., 346, 121-132.
Shukor, M.Y., Dahalan, F.A., Salvamani, S., Jusoh, A.Z., Shamaan, N.A. and Syed, M.A. (2013). Characterization of a diesel-degrading enzymes from Acinetobacter sp. strine DYR12. Bioreme Sci Technol Res. 76(2), 34-45.
Tebyanian, H., Hassanshahian, M., Kariminik, A. (2013). hexadecane-degradation by Teskumurella and Stenotrophomonas strains iso lated from hydrocarbon contaminated soils. Jundishapur. J. Microbiol. 26 (7), e9182.
Zanaroli, G., Toro, S.D., Todaro, D., Varese, G.C., Bertolotto, A. and Fava, F. (2010). Characterization of two diesel fuel degrading microbial consortia enriched from a non-acclimated, complex source of microorganisms. Microb. Cell Factories, 9, 10-18.
Pollution
Volume 2, Issue 3
July 2016
Pages 365-374

Files

Share

How to cite

Statistics