Assessment of water quality in Halda River (the Major carp breeding ground) of Bangladesh

Document Type : Original Research Paper

Authors

1 Institute of Marine Sciences and Fisheries, University of Chittagong, Chittagong, Bangladesh

2 Bangladesh Council of Scientific and Industrial Research, Chittagong, Bangladesh

10.7508/pj.2017.03. 008

Abstract

The present study has been conducted to assess the surface water quality of Halda River from September 2015 to March 2016. DO, BOD5, COD, pH, EC, Chloride, Alkalinity, and Hardness concentrations in water samples have been found to range within 0.93-5.15 mg/L, 30-545 mg/L, 43-983 mg/L, 6.3-7.3, 110-524 uS/cm, 12-56 mg/L, 35-67 mg/L, and 38-121 mg/L, respectively. Multivariate statistical analyses, such as Principal Component Analysis (PCA) as well as Correlation Matrix (CM) have revealed significant anthropogenic pollutant intrusions in water. Cluster Analysis (CA) has indicated decent results of rendering three different groups of resemblance between the two sampling sites, reflecting the different water quality indicators of the river system. A very strong positive linear relation has been found between COD and BOD (1.000), hardness and EC (0.993), pH and DO (0.979), hardness and COD (0.929), hardness and BOD (0.924), EC and COD (0.922), and EC and BOD (0.916) at a significance level of 0.01, proving their common origin entirely from industrial effluents, municipal wastes, and agricultural activities. River Pollution Index (RPI) has indicated that the water from rivers at Kalurghat and Modhunaghat varied from low to high pollution, which is due to the former area's being mostly industrial zone with some domestic sewage, while the latter underwent less industrial activities. On the contrary, lots of agricultural activities have been found in Modhunaghat. Use of river water can pose serious problems to human health and aquatic ecosystem via biological food chain. The present research suggests special preference for proper management of the river with eco-friendly automation along with development of the country's sustainable economic.

Keywords


INTRODUCTION

Bangladesh, basically known as land of rivers, is filled with 700 rivers including tributaries (Chowdhury, 2001). Halda River, being the only tidal river of Bangladesh, serves as a natural source of fertilized carp eggs (Saimon et al., 2016). Indian Major Carps are spawn naturally in Halda River in Bangladesh, making this river a unique heritage of the country (Tsai et al., 1981; Patra & Azadi, 1985; Kabir et al., 2013). Generally, Indian Major Carp, i.e. Rohu (Labeo rohita), Katla (Gibelion catla), and Mrigal (Cirrhinus cirrhosus), are collected by fishermen from Halda River that hugely support their daily lives (Tsai et al., 1981). Water is the most essential and important compound for all living creatures that form ecosystems (Kataria et al., 2011; Manjare et al., 2010) and river water is used for innumerable rural and urban communities and livestock, fish culture, recharge of ground water, control of floods etc. (Gurunathan & Shanmugam, 2006). However, water quality is being degraded unceasingly due to haphazard industrialization (Manjare et al., 2010). Chemicals, produced from industries, are a major source of water pollution that, carried through geological materials, may cause problems (Kataria et al., 2011). Most of the industries are major contributor of trace metals with the rivers playing the vital role in carrying off municipal and industrial wastewater (Singh et al., 2004; Singh et al., 2005; Wang et al., 2007). Chemical, physical, and biological contaminants from anthropogenic activities pollute surface water. River pollution is a matter of concern all over the world (May et al., 2006; Noori et al., 2010a; Ouyang et al., 2010). At first polluted river affect its chemical quality of water, then to destroy the community structure steadily, disrupting the subtle food web (Joshi et al., 2009). Most probably the effects of industrialization are in the state of beggar description on both aquatic biota and human being. According to WHO, up to 80% of all illnesses and ailments in the world is triggered by poor sanitation and contaminated water (Budhathoki, 2010). Bangladesh is facing serious problems with water contaminations from different industries, domestic wastes, and agrochemicals (Venugopal et al., 2009; Islam et al., 2015a, b). Water quality can be assessed by its physical, chemical, and biological properties (Manjare et al., 2010). RPI has been used in analyses of water quality (Chen et al., 2012; Lai et al., 2013; Hoseinzadeh et al., 2014; Rim-Rukeh, 2016). Moreover, many scholars have used CA, PCA, and Factors Analysis (FA) to evaluate and categorize water quality. Wang et al. (2014) used these statistical analyses for correct interpretation of the water chemistry. Furthermore, Singh et al. (2004), Kowalkowski et al. (2006), Qadir et al. (2007), Venkatesharaju et al. (2010), Noori et al. (2011), Wang et al. (2012), Selle et al. (2013), Jiang-Qi et al. (2013), and Talukder et al. (2016) stated that multivariate statistical methods can be very effective tools to interpret the complex data sets easily and clearly, recognizing pollution factors and assessing water quality parameters with spatio-temporal deviation.

Therefore, trustworthy information on the characteristics of water quality is direly needed so as to control pollution effectively and manage sustainable water resources. As a consequence, it is really necessary to assess river water. The present study has been conducted to assess the water quality and provide the baseline data of the area, which will be useful to measure any anthropogenic pollution level.

MATERIALS AND METHODS

Water samples were collected form the two sites of Kalurghat and Modhunaghat along Halda River (Fig. 1). Sampling procedures were performed in three phases: first in September 2015 (rainy season), second in January 2016 (winter season), and third in March 2016 (pre-monsoon). 

Fig. 1. Map showing the sampling sites of the study area

After selecting the sampling sites, a total of 12 water samples were collected, six from Kalurghat and six from Modhunaghat. Two liters of surface water sample were regularly collected in the morning, between 10 to 11am, in polythene bottle for every seasons. Immediately after collection, water sample were transferred to the laboratory of Bangladesh Council of Scientific and Industrial Research (BCSIR), Chittagong, where pH and dissolved oxygen were measured.

The value of Hydrogen-ion-Concentration (pH) of water was determined by using Hanna pH meter, while chloride was measured by Volhard Method, hardness by Compleximetric Method, alkalinity by Titrimatric, EC by Hanna EC meter, Dissolve Oxygen (DO) by DO meter (Hanna DO meter, HI-9146), Bio-chemical Oxygen Demand (BOD) by Modified Winkler Method (1988), and Chemical Oxygen Demand (COD) by Dichromate Reflux Method stated by APHA (2005).

One Way Analysis of Variance (Post-hoc LSD test) was done to show the variations in concentrations of water parameters in terms of seasons and sites, using SPSS (v.22). According to Dreher (2003), Principal Component Analysis (PCA) was performed on the original data set (without any weighting or standardization). PCA was executed to sort out the principle features of variations in dataset with simplification and classification of raw data. Noori et al. (2008) used principal component analysis (PCA) technique with the SVM to forecast the weekly generated waste in the city of Mashhad, reporting that it was a potential tool to predict waste generation. Noori et al. (2010a) used PCA technique to analyze surface water quality of the Karoon River, Iran. And Noori et al. (2010b) used PCA to predict monthly stream flow of the river.

PCA delivers strategies on spatial and temporal distribution of resultant factors. Cluster Analysis (CA) is an effective tool to find out the similarity and dissimilarity of the influencing factors on different data sets (Wang et al., 2014). Moreover, CA is an important tool for characterization and simplification of datasets, based on their behavior. According to Singh et al. (2004). Pearson’s product moment correlation matrix was done to identify the relation among parameters to strengthen the results from multivariate analysis. CA (Dendogram) was performed to show the similarity among the variables and identify their sources of origin, using PRIMER (v.6).

Each water quality variable used to determine River Pollution Index (RPI) was changed to one of four index scores (Si= 1, 3, 6, or 10). Particularly, RPI referred to arithmetic average of these index scores, with respect to water quality.

The River Pollution Index (RPI) is computed, using the following equation (Liou et al., 2006).

 

where Si represents the index scores and RPI value ranges from 1 to 10.

Table 1. River Pollution Index (RPI) Chart (Chen et al., 2012; Liou et al., 2006)

Items/ ranks

Good

Less polluted

Moderately polluted

Highly polluted

DO (mg/L)

>6.5

4.6-6.5

2.0-4.5

<2.0

BOD5 (mg/L)

<3.5

3.0-4.9

5.0-15

>15

SS (mg/L)

<2.0

20-49

50-100

>100

NH3-N (mg/L)

<0.5

0.5-0.9

91.0-3.0

>3.0

Index scores (Si)

1

3

6

10

Sub-index

<2

2.0-3.0

3.1-6.0

>6.0

 

RESULTS AND DISCUSSION

Dissolved Oxygen (DO) is the amount of oxygen, dissolved in the water (Effendi et al., 2015). Both in natural and waste water; the physical, chemical, and biological activities regulate DO levels (Huq & Alam, 2005). Natural water bodies demonstrate high levels of oxygen which vary depending on temperature, salinity, water turbulence, and atmospheric pressure (Effendi, 2003). Cold water hold more oxygen than warm one (Said et al., 2004). Various biological life forms are greatly influenced by the DO level (Saksena et al., 2008). The survival of aquatic organisms largely depends on the oxygen dissolved in water. The low DO directs high demand for oxygen by the microorganisms (Ott, 1978).  In the present study, the dissolve oxygen was found between (0.93-5.15) mg/L in sampling site. The highest DO was recorded 5.15 mg/L at Kalurghat during post-monsoon and the lowest was recorded 0.93 mg/L during monsoon at Modhunaghat (Table 2). More or less, similar observations have also been recorded by Effendi et al. (2015), Gasim et al. (2007), Alam et al. (1996), Jashimuddin and Khan (1993), Hossain and Khan (1992), Islam and Khan (1993), Hossain et al. (1988), Bhuyian (1979), Khan et al. (1976), and Mahmood and Bhuyian (1988).

 

Table 2. Chemical parameters of water at two sampling points during the three seasons

Parameters/Sites

Seasons

DO (mg/L)

BOD5          (mg/L)

COD         (mg/L)

pH

EC  (uS/cm)

Chloride   (mg/L)

Alkalinity (mg/L)

Hardness  (mg/L)

Kalurghat

Pre-Monsoon

5.10

31

43

7.1

110

35

63

43

Modhunaghat

4.7

32

59

7.2

123

12

65

40

Kalurghat

Monsoon

1.03

339

595

6.3

189

15

35

58

Modhunaghat

0.93

545

983

6.4

524

56

67

121

Kalurghat

Post- Monsoon

5.15

 

30

50

7.2

112

36

65

46

Modhunaghat

4.90

 

35

65

7.3

125

15

67

38

 

Biochemical Oxygen Demand (BOD) is the amount of oxygen used by microbes to decay carbon-based materials in water within a five-day period (APHA, 2005). Low BOD in water indicates that the riverside is free from organic pollution (Saksena et al., 2008) while high BOD is detrimental as it reduces the DO (Fatoki et al., 2005). Paul (1999) mentioned that, river water with a BOD rate above 10mg/L is considered to be moderate, while above 20 mg/L is considered highly-contaminated water.

The BOD in Halda River ranging 30-545 mg/L was relatively high (Table 2). Lower amount of BOD has been observed by Kataria et al. (2011) at Bhopal city water and by Sikder et al. (2016) at the Turag River.

Fig. 2. G-graph showing the concentrations of water quality parameters during different seasons

 

Chemical Oxygen Demand (COD) is a reliable factor for judging the pollution degree in water (Loomer & Cooke, 2011). Higher COD is harmful to all aquatic living organisms, which increase pollution in water bodies (Nian et al., 2007). COD in pure water must be below 20 mg/L, whereas water bodies with a COD above 200 mg/L is considered contaminated (Effendi et al., 2015).

COD varied from 43 to 983 mg/L in the present study (Table 2), which is far above the results, found by Sikder et al., (2016), Ahmed and Nizamuddin (2012), and Miah (2012).

Roy (1955), Moore (1972), APHA (2005), Mahmood and Bhuyian (1988), Sarma et al. (1982), and Campbell (1978) stated that industrial or municipal waste materials played a significant role in increasing or decreasing pH of the adjacent water body, into which waste materials had been dumped. Moreover, activities like bathing, washing, and latrines along water bodies related to pH fluctuations (Effendi et al., 2015). Air temperature is the primary responsible factor for changing the pH of water. Furthermore, bio-chemical and chemical reactions are influenced by the pH (Manjare et al., 2010). This report is thoroughly in accord with the study by Farshad and Venkataramana (2012).

In the present study, results indicate that the water of the river was slightly alkaline (6.3-7.3) in nature (Table 2). In surface water the standard value of pH ranged within 6.5-8.5 (ECR, 1997). Ahmed and Rahman (2000) mentioned that in most raw water sources, pH lies within the range of 6.5- 8.5.

Fig. 3. G-graph showing the concentrations of water quality parameters at different sites

 

Electrical Conductivity (EC) is usually used to indicate the total concentration of ionized constituents of water (Huq & Alam, 2005). A higher conductivity reflects higher water pollution (Florescu et al., 2010). Typical EC value is 300 μS/cm (De, 2007). In the present study, EC ranged between 110 and 524 uS/cm. The highest value (524 uS/cm) was found at Modhunaghat during monsoon, while the lowest one (110 uS/cm) was recorded at Kalurghat during pre-monsoon (Table 2). Hoque et al. (2012) recorded the mean values of EC to be 452.4 μs/cm in monsoon and 901 μS/cm in winter, in the Bansi River. Florescu et al. (2010) found 237 μS/cm (on Arges River) and 960 μS/cm (on Olt River) from southern Romania.

Generally, chloridesare not injurious to public health, though the sodium part of Nacl salt is connected to heart and kidney diseases (Florescu et al., 2010). Sodium chloride (Nacl) may render a salty taste at 250 mg/L. Elevated concentrations of dissolved salts in water compromise its use for domestic or agricultural purposes. Excessive amount of Cl- in inland water is usually regarded as an index of pollution and can be found across hygienic and industrial waters (Florescu et al., 2010).

In the present study the concentrations of chlorides were recorded between 12 and 56 mg/L, with the maximum amount found at Modhunaghat during monsoon and the minimum value (10 mg/L) at Modhunaghat during pre-monsoon (Table 2).

In the present study alkanity ranged from 35 to 67 mg/L. The highest concentration (67 mg/L) belonged to Modhunaghat during monsoon and post-monsoon, whereas the lowest amount (35 mg/L) was found at Kalurghat during monsoon (Table 2). Hoque et al. (2012) reported that the value of alkalinity in monsoon and winter seasons were 50.4 mg/L and 146.5 mg/L in the Bansi River, respectively.

Hardness depends on the presence of Mg and Ca ions in water. The richness of Ca relies on its natural occurrence in the earth’s crust. River waters usually contain (1-2) mg/L calcium, but in lime river zones water can contain as high as 100 mg/L calcium. Ca ion exerts great influence on aquatic organisms, enhancing metal toxicity in their gills (Florescu et al., 2010). In the present study hardness was recorded between 38 and 121 mg/L. Maximum concentration of hardness (121 mg/L) was found at Modhunaghat during monsoon and minimum concentration of this parameter (38 mg/L) was recorded at Modhunaghat during post-monsoon season (Table 2). Joshi et al. (2009) recorded higher hardness in the monsoon season (120.62 mg/L) and lower in the winter season (87.55 mg/L) in the River Ganga.

Significant variations were found for DO, BOD, COD, and pHin terms of seasons (P<0.05); however, Chloride, Alkalinity, Hardness, and EC showed nothing of this sort with seasons (P>0.05). BOD, COD, pH, Chloride, Alkalinity, Hardness, and EC exhibited no prevalent variations with sites (P>0.05), with the exception of DO (P<0.05, Fig. 4).

Fig. 3. G-graph showing the concentrations of water quality parameters at different sites

 

Electrical Conductivity (EC) is usually used to indicate the total concentration of ionized constituents of water (Huq & Alam, 2005). A higher conductivity reflects higher water pollution (Florescu et al., 2010). Typical EC value is 300 μS/cm (De, 2007). In the present study, EC ranged between 110 and 524 uS/cm. The highest value (524 uS/cm) was found at Modhunaghat during monsoon, while the lowest one (110 uS/cm) was recorded at Kalurghat during pre-monsoon (Table 2). Hoque et al. (2012) recorded the mean values of EC to be 452.4 μs/cm in monsoon and 901 μS/cm in winter, in the Bansi River. Florescu et al. (2010) found 237 μS/cm (on Arges River) and 960 μS/cm (on Olt River) from southern Romania.

Generally, chloridesare not injurious to public health, though the sodium part of Nacl salt is connected to heart and kidney diseases (Florescu et al., 2010). Sodium chloride (Nacl) may render a salty taste at 250 mg/L. Elevated concentrations of dissolved salts in water compromise its use for domestic or agricultural purposes. Excessive amount of Cl- in inland water is usually regarded as an index of pollution and can be found across hygienic and industrial waters (Florescu et al., 2010).

In the present study the concentrations of chlorides were recorded between 12 and 56 mg/L, with the maximum amount found at Modhunaghat during monsoon and the minimum value (10 mg/L) at Modhunaghat during pre-monsoon (Table 2).

In the present study alkanity ranged from 35 to 67 mg/L. The highest concentration (67 mg/L) belonged to Modhunaghat during monsoon and post-monsoon, whereas the lowest amount (35 mg/L) was found at Kalurghat during monsoon (Table 2). Hoque et al. (2012) reported that the value of alkalinity in monsoon and winter seasons were 50.4 mg/L and 146.5 mg/L in the Bansi River, respectively.

Hardness depends on the presence of Mg and Ca ions in water. The richness of Ca relies on its natural occurrence in the earth’s crust. River waters usually contain (1-2) mg/L calcium, but in lime river zones water can contain as high as 100 mg/L calcium. Ca ion exerts great influence on aquatic organisms, enhancing metal toxicity in their gills (Florescu et al., 2010). In the present study hardness was recorded between 38 and 121 mg/L. Maximum concentration of hardness (121 mg/L) was found at Modhunaghat during monsoon and minimum concentration of this parameter (38 mg/L) was recorded at Modhunaghat during post-monsoon season (Table 2). Joshi et al. (2009) recorded higher hardness in the monsoon season (120.62 mg/L) and lower in the winter season (87.55 mg/L) in the River Ganga.

Significant variations were found for DO, BOD, COD, and pHin terms of seasons (P<0.05); however, Chloride, Alkalinity, Hardness, and EC showed nothing of this sort with seasons (P>0.05). BOD, COD, pH, Chloride, Alkalinity, Hardness, and EC exhibited no prevalent variations with sites (P>0.05), with the exception of DO (P<0.05, Fig. 4).

Fig. 5. Dendogram showing the percentage of similarity among water parameters during different seasons(BOD= Bio-chemical oxygen demand; COD= Chemical oxygen demand; EC=Electrical conductivity; and DO= Dissolve oxygen)

                      

CA was carried out, using square root and Bray Curtis Similarity, to show the similarity among the parameters that hugely contribute to water pollution. From the output of the cluster analysis, three clusters were found during different seasons: Cluster 1, including DO and pH; Cluster 2, including EC, COD, and BOD5; and Cluster 3, including Chloride, Alkanity, and Hardness (Fig. 5). DO and pH represent a strong connection with minimum cluster distance, indicating that these parameters are influential during seasonal variations. Parameters are grouped together in less distance have higher affinity with similar identical behavior, during temporal variations. They also exert a probable effect on each other. Furthermore EC, COD, and BOD5 also have strong linkage, though lesser than cluster 1. However, they contribute largely to the environmental process. Chloride, Alkanity, and Hardness are under the group of cluster 3 with minimum distance, compared to cluster 1 and cluster 2. Nonetheless they affect the environment. Impacted site is the effluent-discharged area of the river, highly influenced by untreated industrial effluents, agricultural inputs, and domestic wastes.

In river water environment, the inter-linkage among water parameters deliver noteworthy information as well as pathways for parameters. Results of correlation between water parameters fully consented with those, obtained by PCA and CA, that approve some new associations among the variables. A very strong positive linear relation was found between COD vs BOD (1.000), Hardness vs EC (0.993), pH vs DO (0.979), Hardness vs COD (0.929), Hardness vs BOD (0.924), EC vs COD (0.922), and EC vs BOD (0.916) at a significance level of 0.01 (Table 3).

Strong positive correlations were recorded between Hardness vs Chloride (0.769) and Chloride vs EC (0.718) at the significance level of 0.05 (Table 3). The very strong and strong correlations indicate that the parameters were originated from similar sources, particularly from industrial effluents, domestic wastes, and agricultural inputs. Besides, strong negative correlations were found between DO vs BOD (-0.958), COD vs DO (-0.954), pH vs BOD (-0.922), and pH vs COD (-0.915) in river water.

Table 3. Correlation matrix of chemical parameters in river water

 

DO

BOD

COD

pH

EC

Chloride

Alkalinity

Hardness

DO

1

 

 

 

 

 

 

 

BOD

-0.958

1

 

 

 

 

 

 

COD

-0.954

1.000

1

 

 

 

 

 

pH

0.979

-0.922

-0.915

1

 

 

 

 

EC

-0.768

0.916

0.922

-0.698

1

 

 

 

Chloride

-0.297

0.532

0.534

-0.323

0.718

1

 

 

Alkalinity

0.561

-0.314

-0.299

0.643

0.091

0.381

1

 

Hardness

-0.777

0.924

0.929

-0.728

0.993

0.769

0.054

1

 

Subsequently Principal Component Analysis (PCA) was carried out, in which the components were regarded as principal components whose Eigen values were above 0.5. Principal component highlighted the most vital factors that affect the water quality of the study area. PC1 had an initial Eigen value of 7.948 and a total variance of 99.349%, with strong positive loading of DO, pH, Chloride, and Alkalinity that resembled the loading of pollution, mainly caused by untreated organic load with crucial anthropogenic effect (Table 4). PC 1 had high negative loading of BOD, COD, EC, and Hardness. It can be represented as the effect of geological changes on environmental parameters. From the present PCA study, it may be concluded that the source of PC 1 can originate from a mixed source of anthropogenic inputs, particularly from industrial wastes and agricultural actions in the study area.

Table 4. Component matrix of factors model with strong loadings in river water

Component Matrix

Eigen value (0.5)

Component1

DO

1.000

BOD

-1.000

COD

-1.000

pH

0.995

EC

-1.000

Chloride

0.999

Alkalinity

0.998

Hardness

-0.982

Eigen value

7.948

% Total variance

99.349

Cumulative %

99.349

 

 

 

 

Recently, the simple method of River Pollution Index (RPI) has been used concurrently by different organization, like Taiwan EPA, to assess the surface water quality. This method comprises with concentration level of four parameters, namely DO, BOD, SS, and NH3-N. Pollution status is calculated using four-state of each parameter.

The current study compared the concentrations of DO and BOD5 with those of RPI table, in order to weigh the status of particular water variables (Table 1). Average DO in the Kalurghat turned out to be 1.03 mg/L during monsoon season, indicating that the water was highly polluted in comparison with RPI. The water of Modhunaghat contained 0.93 mg/L DO, indicating that the water was highly polluted, too.

Averages DO in the Kalurghat was recorded 5.15 mg/L during post- monsoon season which can be treated as less polluted zone according to RPI index, while 4.90 mg/L DO was found from the Modhunaghat, suggesting the area was less polluted. During pre-monsoon the average DO concentration was 5.1 mg/L at Kalurghat, which designated less pollution in this area, according to RPI table, where 4.7 mg/L was found from Modhunaghat area, also indicative of an area, characterized with less pollution.

Average amount of BOD5 were found between 31 and 545 mg/L for all seasons at Kalurghat and Modhunaghat, indicating that the water of the river was highly polluted, according to Table 1.

 

CONCLUSION

Findings of this study indicate that the water of the river is being polluted gradually. Unplanned industrialization, urbanization, and agricultural activities are the prime responsible factors for continuous pollution of river water. River water cause great harm to human beings if it is used for domestic, irrigation, and pisciculture purposes. Moreover, the excessive concentrations of some water parameters can pose great risk to fish and human communities, dwelling in and adjacent to Halda River. Finally, this research gives special focus to water quality assessment for better management of the river water to protect the health of aquatic ecosystem of the river.

Acknowledgements

The authors are thankful to the Bangladesh Council of Scientific and Industrial Research (BCSIR), Chittagong for giving the facility to conduct tests of water quality parameters. 

Ahmed, M.F. and Rahman, M.M. (2000). Water supply and sanitation. ITN-Bangladesh, Centre for Water Supply and Waste Management, BUET, Dhaka, Bangladesh.
Ahmed, M.J. and Nizamuddin, M. (2012). Physicochemical assessment of textile effluents in Chittagong Region of Bangladesh and their possible effects on environment. Inter. J of Res. in Chemis. Environ., 2, 220-230.
Alam, M.M., Islam, M.A., Islam, S. and Haider, S.Z. (1996). Surface water quality of various polluted locations. J Bangladesh Chemi. Soci., 8, 129-137.  
APHA (American Public Health Association) (2005). Standard methods for the examination of water and waste water. 21st ed American Public Health Association (APHA). Washington DC: USA Port City Press.
Bhuyian, A.M. (1979). Effect of industrial pollution on the biology of the Karnafully River. M. Phil Thesis, Deparment of Zoology, University of Chittagong, 164 pp.
Budhathoki, R. (2010). Analysis of the physico-chemical and bacteriological parameters of bottled water available in Kathmandu valley, A Case Study on the Partial Fulfillment of the Requirements for M.Sc., First Year, Environment Science, T.U.  
Campbell, I.C. (1978). A biological investigation of an organically polluted urban stream in Victoria. Austr. J of Mari. Fresh. Res., 29, 275-91. 
Chen, Y.C., Yeh, H.C. and Wei, C. (2012). Estimation of river pollution index in a tidal stream using Kriging analysis. Int. J. Environ. Res. Public Heal., 9, 3085-3100. doi:10.3390/ijerph9093085
Chowdhury, Q.M. (2001). Water and environment. Forum of Environmental Journalist of Bangladesh, ISBN- 983-756-004-8.
De, A.K. (2007). Environmental Chemistry. New age international limited. New Delhi.
Dreher, T. (2003). Evaluation of graphical and multivariate methods for classification of water chemistry data. Hydro. J., 11, 605-606.
ECR (1997). The Environment Conservation Rules. Government of the People’s Republic of Bangladesh. Ministry of Environment and Forest, 205-207.
Effendi, H., Romanto. and Wardiatno, Y. (2015). Water quality status of Ciambulawung River, Banten Province, based on pollution index and NSF-WQI. Procedia Environmental Sciences, 24, 228-237. doi: 10.1016/j.proenv.2015.03.030.
Effendi, H. (2003). Water quality analysis for aquatic resources management. Yogyakarta, Kanisius.
Fatoki, O.S., Muyima, N.Y.O. and Lujiza, N. (2001). Situation analysis of water quality in Umtata River catchment. Water SA, 27, 467-74.
Farshad, H. and Venkataramana, G.V. (2012). Impact of Physico-Chemical Parameters of Water on Zooplankton Diversity in Nanjangud Industrial Area, India. Inter. Res. Journal of Environ. Sci., 1, 37-42.
Florescu, D., Ionete, R.E., Sandru, C., Iordache, A. and Culea, M. (2010). The influence of pollution monitoring parameters in characterizing the surface water quality from Romania southern area. Rom. Journ. Phys., 56. 1001-1010.
Gasim, M.B., Ismail, B.S., Toriman, E., Mir, S.I. and Chek, T.C. (2007). A Physico-chemical Assessment of the Bebar River, Pahang, Malaysia. Glo. J Environ. Res., 1, 7-11.
Gurunathan, A. and Shanmugam, C.R. (2006). Customary rights and their relevance in modern tank management: Select cases in Tamil Nadu, Paper prepared for the workshop entitled ‘water. Law and the Commons’ organized in Delhi from 8 to 10 December  by the International Environmental Law Research Centre (ILERC).
Hoque, M.M.M., Roy, S., Hoque, M.N. and Isla, M.Z. (2012). Assessment of some water quality parameters of Bansi River in Monsoon and winter seasons. J. Environ. Sci. & Natural Resources, 5, 53-57.
Hossain, M.M., Mahmood, N. and Bhuyian, A.L. (1988). Some water quality characteristics of the Karnafuli River estuary. J. of the Asiatic soci. Bang., 21, 183-188. 
Hossain, T. and Khan, Y.S.A. (1992). Study on the Environment Impact Assessment (EIA) of the effluent discharge of the Chittagong Urea Fertilizer Limited (CUFL) on the Karnafuli River estuary. M.Sc. Thesis, Institute of Marine Sciences, University of Chittagong.
Huq, S.M.I. and Alam, M.D. (2005). A handbook on analysis of soil, plant and water. BACER-DU. University of Dhaka, Bangladesh. pp. xxii-246.
Islam, M.S., Ahmed, M.K., Habibullah-Al-Mamun, M. and Hoque, M.F. (2015a). Preliminary assessment of heavy metal contamination in surface sediments from a river in Bangladesh. Environ. Earth Sci., 73, 1837–1848. DOI: 10.1007/s12665-014-3538-5.
Islam, M.S., Ahmed, M.K., Raknuzzaman, M., Habibullah-Al-Mamun, M. and Islam, M.K. (2015b). Heavy metal pollution in surface water and sediment: a preliminary assessment ofan urban riverin a developing country. Ecolog. Indica., 48, 282-291. doi:10.1016/j.ecolind.2014.08.016.
Islam, M.M. and Khan, Y.S.A. (1993). Study on the influence of the effluent discharged by the KPM and KRC on the physiochemical parameters of the Karnafully River, Chittagong. M.Sc. Thesis, Institute of Marine Sciences, University of Chittagong.
Jashimuddin, S.M. and Khan, Y.S.A. (1993). Environmental Impact Assessment (EIA) of the municipal sewage discharge through majhirghat canal on the Karnafuli River water, Chittagong. M. Sc. Thesis, Institute of Marine Sciences, University of Chittagong.
Jiang-Qi, Q., Qing-Jing, Z., Pan, L., Cheng-Xia, J. and Mu, Y. (2013). Assessment of water quality using multivariate statistical methods: A case study of an urban landscape water, Beijing. Int. J. Biosci. Biochem. Bioinforma., 3, 196-200.
Joshi, D.M., Kumar, A. and Agrawal, N. (2009). Studies on physicochemical parameters to assess the water quality of River Ganga for drinking purpose in haridwar district. Rasayan Journal of Chemistry, 2, 195-203.
Kabir, M.H., Kibria, M.M., Jashimuddin M. and Hossain, M.M. (2013). Economic valuation of tangible resources from Halda-the carp spawning unique river located at southern part of Bangladesh. Int. J. Water Res., 1, 30-36.
Kataria, H.C., Gupta, M., Kumar, M., Kushwaha, S., Kashyap, S., Trivedi, S., Bhadoriya, R. and Bandewar, N.K. (2011). Study of physico-chemical parameters of drinking water of Bhopal city with reference to health impacts. Current W. Environ., 6, 95-99.
Khan, Y.S.A., Mahmood, N., Quader, O., Bhuyian, A.L and Ahmed, M.K. (1976). Studies on the hydrology of the Karnafuli estuary. J. of the Asiatic Soci. Bang., 2, 89-99.
Kowalkowski, T., Zbytniewski, R., Szpejna, J. and Buszewski, B. (2006). Application of chemo metrics in water classification. Water Res., 40, 744-752. http://dx.doi.org/10.1016/j.watres.2005.11.042.
Lai, Y.C., Tu, Y.T., Yang, C.P., Surampalli, R.Y. and Kao, C.M. (2013). Development of a water quality modeling system for river pollution index and suspended solid loading evaluation. Journal of Hydrolo., 478, 89-101. doi: 10.1016/j.jhydrol.2012.11.050.
Liou, S.M., Lo, S.L. and Wang, S.H. (2006). A generalized water quality index for Taiwan. Environ. Monit. Assess., 96, 35-52.  doi:10.1023/B:EMAS.0000031715.83752.a1.
Loomer, H.A. and Cooke, S.E. (2011). Water quality in the Grand River watershed: current conditions & trends (2003–2008). Grand River Conservation Authority.
Mahmood, N. and Bhuyian, A.M. (1988). Some water quality characteristic of the Karnafuli River estuary. Mahassagar Bull. Nati Inst. Sci. Ind., 22, 183-188.
Manjare, S.A., Vhanalakar, S.A. and Muley, D.V. (2010). Analysis of water quality using physico-chemical parameters Tamdalge tank in Kolhapur district, Maharashtra. Inter. J Advan. Biotech. Res., 1, 115-119.
May, A.M., Mutasem, E., Mark, D.S. and John, N.L. (2006). Factors influencing development of management strategies for the Abou Ali River in Lebanon. Sci Total Environ., 362, 15-30. http://dx.doi.org/10.1016/j.scitotenv.2005.09.079.
Moore, P. (1972). Studies on the pollution of the Bhadra River fisheries and Bhadravathi (Mysore state) with industrial effluents. Nat. Inst. Sci. Ind., 22, 132-160.
Miah, M.S. (2012). Cost-effective treatment technology on textile industrial wastewater, in Bangladesh. J. of Che. Eng. 27: 32-36. doi: http://dx.doi.org/10.3329/jce.v27i1.15855.
Nian, Y.G., Xu, Q.J., Jin, X.C., Yan, C.Z., Liu and Jiang, J. (2007). Effects of chitosan on growth of an aquatic plant (Hydrilla verticillata) in polluted waters with different chemicaloxygen demands. J of Environ. Sci., 19, 217.
Noori, R., Abdoli, M.A., Ghasrodashti, A.A. and Ghazizade, M.J. (2008). Prediction of municipal solid waste generation with combination of support vector machine and principal component analysis: A case study of Mashhad. Environmental Progress and Sustainable Energy, 28, 249-258. doi: 10.1002/ep.10317.
Noori, R., Sabahi, M.S., Karbassi, A.R., Baghvand, A. and Taati Zadeh, H. (2010a). Multivariate statistical analysis of surface water quality based on correlations and variations in the data set. Desalination, http://dx.doi.org/10.1016/j.desal.2010.04.053.
Noori, R. Khakpour, A., Omidvar, B. and Farokhnia, A. (2010b). Comparison of ANN and principal component analysis-multivariate linear regression models for predicting the river flow based on developed discrepancy ratio statistic. Expert Syst. with Applicat., 37, 5856-5862. http://dx.doi.org/10.1016/j.eswa.2010.02.020.
Noori, R., Karbassi, A.R., Moghaddamnia, Han, D., Zokaei-Ashtiani, M.H., Farokhnia, A. and Ghafari Gousheh, M. (2011). Assessment of input variables determination on the SVM model performance using PCA, Gamma test, and forward selection techniques for monthly stream flow prediction. Journal of Hydrolo., 401, 177–189. http://dx.doi.org/10.1016/j.jhydrol.2011.02.021.
Ott, W.R. (1978). Environmental indices: theory and practice. Michigan: Ann Arbor Science.
Ouyang, Y., Nkedi-Kizza, P., Wu, Q.T., Shinde, D. and Huang, C.H. (2006). Assessment of seasonal variations in surface water quality. Water Res., 40, 3800-3810. http://dx.doi.org/10.1016/j.watres.2006.08.030.
Patra, R.W.R. and Azadi, M.A. (1985). Limnology of the Halda River. J. NOAMI, 2, 31-38.
Paul, G. (1999). Environment and pollution. Poribes Dushan (Ed), Dasgupta and Company Ltd, India, pp. 323.
Qadir, A., Malik, R.N. and Husain, S.Z. (2007). Spatio-temporal variations in water quality of NullahAik-tributary of the river Chenab, Pakistan. Environ. Monit. Assess., 140, 43-59. doi: 10.1007/s10661-007-9846-4.
Rim-Rukeh, A. (2016). Assessment of water quality in a Tidal River system using River Pollution Index (RPI). Journal of Environ. Sci. and Pollut. Res., 2, 72-75.
Roy, H.K. (1955). Plankton ecology of the River Hoogly in Patna. West Bengal. Eco., 36, 169-175.
Sarma, V.V., Raju, G.R.K. and Babu, T.R. (1982). Pollution characteristics and water quality in the Visakhapatnam harbour. Mahassagar Bull. Nati. Inst. Oce., 15, 15-22. 
Saimon, M.K., Mustafa, M.G., Sarker, B.S., Hossain, M.B. and Rahman, M.M. (2016). Marketing channels of Indian carp fry collected from Halda River and livelihood of the fry traders. Asian J. Agric. Res., 10, 28-37. doi: 10.3923/ajar.2016.28.37.
Said, A., Stevens, D.K. and Sehlke, G. (2004). An innovative index for evaluating water quality in streams. Environmental Management, 34, 406-14. doi: 10.1007/s00267-004-0210-y.
Saksena, D.N., Garg, R.K. and Rao, R.J. (2008). Water quality and pollution status of Chambal River in National Chambal Sanctuary, Madhya Pradesh. Journal of Environmental Biology, 29, 701-10.
Selle, M., Schwientek, M. and Lischeid, G. (2013). Understanding processes governing water quality in catchments using principal component scores. Journal of Hydrolo., 486, 31-38. http://dx.doi.org/10.1016/j.jhydrol.2013.01.030.
Sikder, M.N.A., Huq, S.M.S., Mamun, M.A.A., Hoque, K.A.,Bhuyan, M.S. and Bakar, M.A. (2016). Assessment of physicochemical parameters with its effects on human and aquatic animals giving special preference to effective management of Turag River. J of Environ. Sci. Toxico. Food Tech., 10, 41-51.
Singh, K.P., Malik, A. and Sinha, S. (2005). Water quality assessment and apportionment of pollution sources of Gomti river (India) using multivariate statistical techniques- a case study. Anal Chim Acta., 538, 355-374. http://dx.doi.org/10.1016/j.aca.2005.02.006
Singh, K.P., Malik, A., Mohan, D. and Sinha, S. (2004). Multivariate statistical techniques for the evaluation of spatial and temporal variations in water quality of Gomti River (India)- a case study. Wat. Res., 38, 3980-3992. http://dx.doi.org/10.1016/j.watres.2004.06.011
Talukder, A., Mallick, D., Hasin, T., Anka, I.Z. and Hasan, M.M. (2016). Spatio-temporal variability in hydro-chemical characteristics of coastal waters of Salimpur, Chittagong along the Bay of Bengal. J. Fish., 4, 335-344. doi: http://dx.doi.org/10.17017/jfish.v4i1.2016.104.
Tsai, C.F., Islam, M.N., Karim R. and Rahman, K.U.M.S. (1981). Spawning of major carps in the lower Halda River, Bangladesh. Estuaries, 4, 127-138.
Venkatesharaju, K., Somashekar, R.K. and Prakash, K.L. (2010). Study of seasonal and special variation in surface water quality of Cauvery river stretch in Karnataka. J. Ecol. Nat. Environ., 2, 1-9.
Venugopal, T., Giridharan, L. and Jayaprakash, M. (2009). Characterization and risk assessment studies of bed sediments of River Adyar-An application of speciation study. Inter. J Environ. Res., 3, 581-598.
Wang, Y.B., Liu, C.W., Liao, P.Y. and Lee, J.J. (2014). Spatial pattern assessment of river water quality: implications of reducing the number of monitoring stations and chemical parameters. Environ. Monit. Assess., 186, 1781-1792. doi: http://dx.doi.org/10.1007/s10661-013-3492-9.
Wang, X., Cai, Q., Ye, L. and Qu, X. (2012). Evaluation of spatial and temporal variation in stream water by multivariate statistical techniques: A case study of the Xiangxi River basin, China. Quarter. Inter., 1, 1-8. http://dx.doi.org/10.1016/j.quaint.2012.05.015.
Wang, X.L., Lu, Y.L., Han, J.Y., He, G.Z., Wang, T.Y. (2007). Identification of anthropogenic influences on water quality of rivers in Taihu watershed. J Environ Sci-China, 19, 475-481.doi:10.1016/S1001-0742(07)60080-1.
Winkler, L.W. (1988). The determination of dissolved oxygen in water. Berlin. Deut. Chem. yeas, 21.2845 Nov., 133.