Microbeads in Sediment, Dreissenid Mussels, and Anurans in the Littoral Zone of the Upper St. Lawrence River, New York.

Document Type : Original Research Paper


Department of Environmental Studies, St. Lawrence University, Canton, New York, 13617 United States of America


Global plastic production has exceeded 300 million tons per year (Plastics Europe, 2015). In the marine and freshwater environments, larger plastics abrade and photo-degrade resulting in persistent environmental microplastics that are not effectively removed by existing wastewater treatment plants (WWTPs). The ecological effects of microplastics on the marine environment are poorly understood, with even less attention to freshwater systems. To assess whether microplastics have infiltrated food webs of shallow nearshore ecosystems of the St. Lawrence River, we sampled four sites along the international section of the St. Lawrence River, from Alexandria Bay to Waddington, NY. Twelve sediment samples along with one hundred and forty-nine Dreissenid mussels (Dreissena polymorpha and D. bugensis) were collected from the littoral zone, and forty one road-killed anuran amphibian specimens were collected adjacent to the river.  Sediment subsamples at two of four sediment sampling sites contained plastic micro-particles. No microbeads were detected within any of the Dreissenid mussels or anuran digestive tract samples. The Dreissenids were likely too small to ingest microbeads greater than 35 microns. Microplastics congregating in the littoral zone may pose a threat within the food web through potential ingestion, requiring further methodological development. 


Andrady, A.L. (ed.), (2003). Plastics and the environment. New York: Wiley.
Andrady, A.L. (2011). Microplastics in the marine environment. Mar. Pollut. Bull., 62, 1596–1605.
 Bakir, A., O’Connor, I.A., Rowland, S.J. and Hendriks, A.J. (2016). Relative importance of microplastics as a pathway for the transfer of hydrophobic organic chemicals to marine life.  Environ. Pollut., 219, 56-65.
 Baldwin, A.K., Corsi, S.R. and Mason, S.A. (2016). Plastic debris in 29 Great Lakes tributaries: relations to watershed attributes and hydrology. Environ. Sci. Technol., 50, 10377-10385.
 Besseling, E., Wegner, A., Foekema, E.M., van den Heuvel-Greve, M.J. and Koelmans, A.A. (2013). Effects of microplastic on fitness and PCB bioaccumulation by the lugworm Arenicola marina. Environ. Sci. Technol., 47, 593–600.
 Betts, K. (2008). Why small plastic particles may pose a big problem in the oceans. Environ. Sci. Technol., 42, 8995-8995.
Browne, M.A., Galloway, T. and Thompson, R. (2007).  Microplastic - an emerging contaminant of potential concern?  Integr. Environ. Assess. Manage., 3, 559-561.
 Browne, M.A., Dissanayake, A., Galloway, T.S., Lowe, D.M. and Thompson, R.C. (2008). Ingested microscopic plastic translocates to the circulatory system of the mussel, Mytilus edulis (L.). Environ. Sci. Technol., 42, 5026-5031.
 Browne, M.A., Galloway, T.S. and Thompson, R.C. (2010). Spatial patterns of plastic debris along estuarine shorelines. Environ. Sci. Technol.,44, 3404–3409.
 Browne, M.A., Crump, P., Niven, S.J., Teuten, E., Tonkin, A., Galloway, T. and Thompson, R. (2011). Accumulation of microplastic on shorelines worldwide: sources and sinks. Environ. Sci. Technol.,44, 9175–9179.
 Browne, M.A., Niven, S.J., Galloway, T.S. and Thompson, R.C. (2013). Microplastic moves pollutants and additives to worms, reducing functions linked to health and biodiversity.  Curr. Biol., 23, 2388-2392.
 Castañeda, R.A., Avlijas, S., Simard, M.A. and Ricciardi, A. (20140. Microplastic pollution in St. Lawrence River sediments. Can. J. Fish. Aquat. Sci., 71, 1767–1771.
Claessens, M., Meester, S.D., Landuyt, L.V., Clerck, K.D. and Janssen, C.R. (2011). Occurrence and distribution of microplastics in marine sediments along the Belgian coast. Mar. Pollut. Bull., 62, 2199–2204.
 Colborn, T., Davidson, A., Green, S., Hodge, R., Jackson, C. and Liroff, R. (1990).  Great Lakes, Great Legacy?  Washington, D.C., The Conservation Foundation and the Institute for Research on Public Policy.
 Cole, M. and Galloway, T.S. (2015). Ingestion of nanoplastics and microplastics by Pacific oyster larvae. Environ. Sci. Technol., 49, 14625-14632.
 Cole, M., Lindeque, P., Halsband, C. and Galloway, T.S. (2011). Microplastics as contaminants in the marine environment: a review. Mar. Pollut. Bull., 62, 2588-2597.
Comprehensive Wildlife Conservation Strategy for New York (CWCSNY). Northeastern Lake Ontario- St. Lawrence Basin.
 Costa, M., Ivar do Sul, J., Silva-Cavalcanti, J., Araújo, M., Spengler, Â. and Tourinho, P. (2010). On the importance of size of plastic fragments and pellets on the strandline: a snapshot of a Brazilian beach. Environ. Monit. Assess., 168, 299–304.
 Cozar, A., Echevarria, F., Gonzalez-Gordillo, J.I., Irigoien, X., Ubeda, B., Hernandez-Ledon, S. Palma, A.T., Navarro, S., Garcia-de-Loas, J., Ruiz, A. Fenandez-dePuelles, M.L. and Duarte, C.M. (2014). Plastic debris in the open ocean. P. Natl. Acad. Sci., 111, 10239-10244.
 De Sa. L.C., Luis, L.G. and Guilhermino, L. (2015).  Effects of microplastics on juveniles of the common goby (Pomatoschistus microps): confusion with prey, reduction of the predatory performance and efficiency, and possible influence of developmental conditions.   Environ. Pollut., 196, 39-362.
 Derraik, J.G. (2002). The pollution of the marine environment by plastic debris: a review. Mar. Pollut. Bull., 44, 842-852.
 Driedger, A.G.J., Dürr, H.H., Mitchell, K. and Van Cappellen, P. (2015). Plastic debris in the Laurentian Great Lakes: a review. Mar. Pollut. Bull., 41, 9-19.
 Eerkes-Medrano, D., Thompson, R.C. and Aldridge, D.C. (2015). Microplastics in freshwater systems: a review of the emerging threats, identification of knowledge gaps and prioritization of research needs. Water. Res., 75, 63-82.
Environmental Protection Agency. (2007). Report to Congress: Combined Sewer Overflows to the Lake Michigan Basin, EPA-833-R-07-007.
 Eriksen, M., Mason, S., Wilson, S., Box, C., Zellers, A., Edwards, W., Farley, H. and Amato, S. (2013). Microplastic pollution in the surface waters of the Laurentian Great Lakes. Mar. Pollut. Bull., 77, 177-182.
Food and Drug Administration. (2015)0. The Microbead-Free Waters Act: FAQs.  FDA.gov/laws and regulations. Accessed 15 Feb. 2018.
 Fendall, L.S. and Sewell, M.A. (2009).  Contributing to marine pollution by washing your face: Microplastics in facial cleansers. Mar. Pollut. Bull., 58, 1225-1228.
 Free, C.M., Jensen, O.P., Mason, S.A., Eriksen, M., Williamson, N.J. and Boldgiv, B. (2014). High-levels of microplastic pollution in a large, remote, mountain lake. Mar. Pollut. Bull., 85, 156-163.
Gibbs, J.P., Breisch, A.R., Ducey, P.K., Johnson, G., Behler, J. and Bothner, R. (2007). Bullfrog (pp. 132-136) and Northern leopard frog (pp. 141-144) (in: The amphibians and reptiles of New York State: identification, natural history, and conservation. Oxford University Press.)
Herzke, D., Anker-Nilssen, T., Haugdahl Nost, T., Gotsch, A., Chirstensen-Dalsgaard, S., Langset, M., Fangel, K. and Koelmans, A.A. (2016). Negligible impact of ingested microplastics on tissue concentrations of persistent organic pollutants in northern fulmars off coastal Norway. Environ. Sci. Techon., 50, 1924-1933.
 Imhof, H.K., Ivleva, N.P., Schmid, J., Niessner, R., and Laforsch, C. (2013). Contamination of beach sediments of a subalpine lake with microplastic particles. Curr. Biol., 23, R867–R868.
 Johns, C. (2011). Trends of total cadmium, copper, and zinc in the zebra mussel (Dreissena polymorpha) along the upper reach of the St. Lawrence River: 1994—2005. Environ. Monit. Assess., 184, 5371-5385.
 Kalčíková, G., Alič, B., Skala, T., Bundschuh, M. and Žgajnar Gotvajn, A. (2017). Wastewater treatment plant effluents as source of cosmetic polyethylene microbeads to freshwater. Chemosphere., 188, 25-31.
 Koelmans, A.A., Bakir, A., Burton, G.A. and Janssen, C.R. (2016). Microplastic as a vector for chemicals in the aquatic environment: critical review and model-supported reinterpretation of empirical studies.  Environ. Sci. Technol., 50, 3315-3326.
 Law, K.L., Moret-Ferguson, S., Maximenko, N.A., Proskurowski, G., Peacock, E.E., Hafner, J. and Reddy, C.M. (2010). Plastic accumulation in the North Atlantic subtropical gyre. Science., 329, 1185–1188.
 Lechner, A., Keckeis, H., Lumesberger-Loisl, F., Zens, B., Krusch, R., Tritthart, M., Glas, M. and Schludermann, E. (2014). The Danube so colourful: a potpourri of plastic litter outnumbers fish larvae in Europe’s second largest river. Environ. Pollut., 188, 177–181.
 Lee, J., Hong, S., Kyung Song, Y., Hee Hong, S., Chang Jang, Y., Jang, M., Won Heo, N., Myung Han, G., Jeong Lee, M., Kang, D. and Joon Shim, W. (2013). Relationship among the abundances of plastic debris in different size classes on beaches in South Korea. Mar. Pollut. Bull., 77, 349-354.
 Mason, S.A., Kammin, L., Eriksen, M., Aleid, G., Wilson, S., Box, C., Williamson, N. and Riley, A. (2016). Pelagic plastic pollution within the surface waters of Lake Michigan, USA.  J. Gt. Lakes Res., 42(4), 753-759.
 Moore, C.J. (2008). Synthetic polymers in the marine environment: a rapidly increasing, long-term threat. Environ. Res., 108, 131-139.
 Moore, C.J., L, G.L. and Zellers, A.F. (2011). Quantity and type of plastic debris flowing from two urban rivers to coastal waters and beaches of Southern California. J. Integr. Coast. Zone Manag., 11, 65-73.
Nalbone, J. (2014). Unseen threat: how microbeads harm New York waters, wildlife, health and environment. Environmental Protection Bureau of the New York State Attorney General's Office, Albany, NY, USA. (15 pp.).
 Peters, C.A. and Bratton, S.P. (2016). Urbanization is a major influence on microplastic ingestion by sunfish in the Brazos River Basin, Central Texas, USA. Environ. Pollut., 210, 380-387.
 Plastics Europe. (2015). Plastics - The Facts 2014/2015; An analysis of European plastics production, demand, and waste data. Brussels, Belgium.
 Ram, J.L., Moore, D., Putchakayala, S., Paredes, A.A., Ma, D. and Croll, R.P. (1999). Serotonergic responses of the siphons and adjacent mantle tissue of the zebra mussel, Dreissena polymorpha. Comp. Biochem. Physiol. Part C., 124, 211-220.
 Rochman, C.M., Kurobe, T., Flores, I. and Teh, S.J. (2014).  Early warning signs of endocrine disruption in adult fish from the ingestion of polyethylene with and without sorbed chemical pollutants from the marine environment.  Sci. Total Environ., 493C, 656-661.
Ryan, P.G., Moore, C.J., van Franeker, J.A. and Moloney, C.L. (2009). Review: Monitoring the abundance of plastic debris in the marine environment. Phil. Trans. R. Soc. B., 364, 1999-2012.
Sanchez, W., Bender, C. and Porcher, J.M. (2014). Wild gudgeons (Gobio gobio) from French rivers are contaminated by microplastics: Preliminary study and first evidence. Environ. Res., 128, 98–100.
Sleight, V.A. Bakir, A., Thompson, R.C. and Henry, T.B. (2016).  Assessment of microplastic-sorbed contaminant bioavailability through analysis of biomarker gene expression in larval zebrafish.  Mar. Pollut. Bull., 116, 291-297.
 Sprung, M. and Rose, U. (1988). Influence of food size and food quantity on the feeding of the mussel Dreissena polymorpha. Oecologia., 77, 526-532.
 Talvitie, J., Mikola, A., Koistinen, A. and Setala, O. (2017). Solutions to microplastic pollution - Removal of microplastics from wastewater effluent with advanced wastewater treatment technologies. Water Res., 123, 401-407.
 Tanaka, K., Takada, H., Yamashita, R., Mizukawa, K., Fukuwaka, M. and Watanuki, Y. (2015). Facilitated leaching of additive derived PBDEs from plastic by seabirds’ stomach oil and accumulation in tissues.  Environ. Sci. Technol., 49, 11799-11807.
 Thompson, R.C., Olsen, Y., Mitchell, R.P., Davis, A., Rowland, S.J., John, A.W., McGonigle, D. and Russell, A.E. (2004). Lost at sea: where is all the plastic? Science., 304, 838-838.
 Teuten, E.L., Rowland, S.J., Galloway, T.S. and Thompson, R.C. (2007). Potential for plastics to transport hydrophobic contaminants. Environ. Sci. Technol. ,41, 7759-7764.
 Todd, B.D. and Winne, C.T. (2006). Ontogenetic and interspecific variation in timing of movement and responses to climatic factors during migration by pond-breeding amphibians. Can. J. Zool., 84, 715-722.
 Twiss, M. (2016). Standardized methods are required to assess and manage microplastic contamination of the Great Lakes system. Commentary. J. Gt. Lakes Res., 42, 921-925.
 Vianello, A., Boldrin, A., Guerriero, P., Moschino, V., Rella, R., Sturaro, A. and Da Ros, L. (2013). Microplastic particles in sediments of Lagoon of Venice, Italy: first observations on occurrence, spatial patterns and identification. Estuar. Coast. Mar. Sci., 130, 54-61.
Wagner, M., Scherer, C., Alvarez-Muñoz, D., Brennholt, N., Bourrain, X., Buchinger, S., Fries, E., Grosbois, C., Klasmeier, J., Marti, T., Rodriguez-Mozaz, S., Urbatzka, R., Vethhaak, A.D., Winther-Nielsen, M. and Reifferscheid, G. (2014). Microplastics in freshwater ecosystems: what we know and what we need to know. Environ. Sci. Europe., 26, 1-9.
 Watts, A.J.R., Lewis, C., Goodhead, R.M., Beckett, S.J., Moger, J., Tyler, C.R. and Galloway, T.S. (2014). Uptake and Retention of Microplastics by the Shore Crab Carinus maenas. Environ Sci Technol, 48, 8823-8830.
 Wright, S.L., Thompson, R.C. and Galloway, T.S. (2013). The physical impacts of microplastics on marine organisms: a review. Environ Pollut, 178, 483–492.
M. and Corcoran, P.L. (2011). Distribution and degradation of fresh water plastic particles along the beaches of Lake Huron, Canada. Water. Air. Soil. Pollut., 220, 365–372.
 Zbyszewski, M., Corcoran, P.L. and Hockin, A. (2014). Comparison of the distribution and degradation of plastic debris along shorelines of the Great Lakes, North America. J. Gt. Lakes Res., 40, 288–299.