Evaluating the effectiveness of Tamarindus indica partially activated seed coat biomass in removing of nitrates from aqueous solutions

Document Type : Original Research Paper


1 Department of Environmental Sciences, Sri Venkateswara University, Tirupati-517 502, Andhra Pradesh, India.

2 Department of Microbiology, Palamuru University, Mahabubnagar-509001, Telangana State, India.


Biomass derived from Tamarindus indica partially activated seed coat was investigated for the removal of nitrate ions from aqueous solutions. Batch experiments were performed to evaluate the parameters like pH, contact time, sorbent dose and initial nitrate concentration. pH of the solution played vital role. The maximum sorption observed at pH=7, sorbent dose 300mg, contact time at 120min, initial nitrate concentration 5mg. Physicochemical properties of the biomass were evaluated using scanning electron microscopy (SEM), energy dispersive X-ray analysis and Fourier Transform infra red (FTIR) spectroscopy. The SEM and FTIR data reveals the suitable surface and the presence of chemical functional groups such as hydroxyl, amide, carbonyl strong acid and primary amine on the biosorbent surface contributes to biosorption. The equilibrium isotherms and kinetics were deliberated. Biosorption equilibrium followed Langmuir isotherm. Pseudo second order kinetics provided better correlation of the experimental data in comparison with pseudo-first-order kinetic model. The study indicated that Tamarindus indica partially activated seed coat biomass found to be a novel biosorbent for the removal of nitrates from aqueous solutions.


Athar, M., Farooq, U., Ali, S.Z. and Salman, M. (2014). Insight into the binding of copper (II) by non-toxic biodegradable material (Oryza sativa): effect of modification and interfering ions. Clean Technol. Environ. Policy., 16; 579–590.
Bailey-Watts, A.E., Gunn, I.M.D. and Kirika, A. (1993). Loch Leven: Past and Current Water Quality and Options for Change. Edinburgh: Report to the Fourth River Purification Board, Institute of Freshwater Ecology
Bhaumik, R., Mondal, N.K., Das, B., Roy, P., Pal, K.C., Das, C., Banerjee, A. and Dutta, J.K. (2012a). Eggshell powder as biosorbent for removal of fluoride from aqueous solution: equilibrium, kinetic and thermodynamic studies. E. J. Chem., 9(3); 1457–1480.
Bhaumik, R., Mondal, N.K., Das, B., Roy, P., Pal, K.C., Das, C., Banerjee, A. and Dutta, J.K.  (2012b). Eggshell powder as an adsorbent for removal of fluoride from aqueous solution: equilibrium, kinetic and thermodynamic studies. E. J. Chem., 9(3); 1457–1480.
Chu, K.H. (2002). Removal of copper from aqueous solution by chitosan in prawn shell: biosorption equilibrium and kinetics. J. Haz. Mat., 90(1); 77–95.
Figucira, M.M.F., Volesky, B. and Cininelli, V.S.T. (2000). Biosorption of metals in brown sea wood biomass. Water Res., 34; 196–204.

Fourest, E. and Roux, J. C. (1992). Heavy metal biosorption by fungal mycelial by-products: mechanisms and influence of pH.  Appl. Microbiol. and Biotech., 37; 399-403.

Freundlich, H.M.F. and Hellen, W. (1939). The adsorption of Cis- and Trans-Azobenzene. J. Am. Chem. Soc., 61; 2228–2230.
Ghafari, S., Hasan, M. and Aroua, M.K. (2008). Bio-electrochemical removal of nitrate from water and wastewater-a review. Bioresour. Technol., 99; 3965-3974.
Hamdi, W., Gamaoun, F., Pelster, D.E.  and Seffen, M. (2013). Nitrate sorption in an agricultural soil profile. Appl. and Environ. Soil Sci., 4(3); 91-98.
Jagruti, N., Sandip, D., Maind. and Satish, A. (2015). Biosorption of Lead (II) and Chromium (VI) Onto Tarminalia Catappa L. Leaves: A Comparative Evaluation.  J. Appli. Chem., 4 (6); 1700-1715.
Jalali, M. (2011). Nitrate pollution of groundwater in Toyserkan, western Iran. Environ. Earth Sci., 62; 907–913.
Kalyan, Y., Mok-Ryun Yu., Hoon Roh., Jae-Kyu Yang. and Yoon-Young Chang. (2013). Buffalo weed (Ambrosia trifida L. var. trifida) biochar for cadmium (II) and lead (II) adsorption in single and mixed system. Desalination and Water Treatment., 51:40-42; 7732-7745.
 Katz. I. and Dosoretz, C.G. (2008). Desalination of domestic wastewater effluents: phosphate removal as pretreatment. Desalination., 222; 230–242.
Knobeloch, L., Salna, B., Hogan, A., Postle, J. and Anderson, H. (2000). Blue Babies and Nitrate Contaminated Well Water. Environmental Health Perspectives Environ. Health Perspect., 108; 675-682.
Langmuir, I. (1918).  Adsorption of gases on plain surfaces of glass, mica and platinum. J. Am. Chem. Soc., 40; 1361–1403.
Mohammad, W., Amer Rafat, A., Ahmad, A.M. and Awwad, M. (2015). Biosorption of Cu(II), Ni(II), Zn(II) and Pb(II) ions from aqueous solution by Sophora japonica pods powder. Int. J. Ind. Chem., 6; 67–75.  
Mousavi, S., Ibrahim, M.K., Aroua, S. and Ghafari, S. (2012). Development of nitrate elimination by autohydrogenotrophic bacteria in bioelectrochemical reactors–A review. Biochem. Eng. J., 67; 251-264.
Mustaqeem, M., Sharif Bagwan, M. and Patil, P. R. (2014). Removal of Pb (II) ion from aqueous Solution Using Potential Low Cost Adsorbents: An Isothermal modeling study. J. Appli. Chem., 3 (1): 275-282.
Nabil Alaa El-Din, M., Madany, I.M., Al-Tayaran, A., Hakeem Al-Jubair, A. and Gomaa, A. (1994). Trends in water quality of some wells in Saudi Arabia, 1984–1989. Sci. Total Environ., 143; 173–181.
Neal, C., Jarvie, H.P., Neal, M., Hill, L. and Wickham, H. (2006). Nitrate concentrations in river waters of the upper Thames and its tributaries. Sci. Total Environ., 365; 15–32.
Nilanjana Das, R., Vimala, K. and Karthika, P. (2008). Biosorption of heavy metals: an overview. Ind. J. Biotech., 7; 159-169.
Nixon, N. (1992). English water utility tackles nitrate removal. Water Eng. Manage., 139(3); 27–28.
Power, J.F. and Schepers, J.S. (1989). Nitrate contamination of groundwater in North America. Agr. Ecosyst. Environ., 26; 165–187.
Sadeq, M., Moe, C.L., Attarassi, B., Cherkaoui, I. and  Idrissi, L. (2008). Drinking water nitrate and prevalence of methemoglobinemia among infants and children aged 1–7 years in Moroccan areas. Int J. Hyg. Environ. Health., 211; 546–554.
Shahanaz Begum, S.A., Tharakeswar, Y., Kalyan, Y. and Ramakrishna Naidu, G. (2015). Biosorption of Cd (II), Cr (VI) & Pb (II) from Aqueous Solution Using Mirabilis jalapa as Adsorbent. J. Encap. and Ads. Sci., 5; 93-104.
Shrimali, M. and Singh, K.P. (2001). New methods of nitrate removal from water. Environ. Pollut., 112; 351–359.
Soumya Bikash, G. (2016).  Optimization study of adsorption parameters for removal of fluoride using aluminium-impregnated potato plant ash by response surface methodology. Clean Techn. Environ. Policy., 18; 1069–1083.
Ramanaiah, S.V., Venkata Mohan, S. and Sarma, P.N.  (2007). Adsorptive removal of fluoride from aqueous phase using waste fungus (Pleurotus ostreatus 1804) biosorbent: Kinetics evaluation. Ecol. Eng., 3 (1); 47–56.
Venkata Mohan, S., Chandrasekhar Rao, N. and Karthikeyan, J. (2002). Adsorption removal of direct azo dye aqueous phase onto coal based sorbents: a kinetic and mechanistic study. J. Hazard. Mater., 90 (2); 189–204.
Xie, Y-X., Xiong, Z-Q., Xing, G-X., Sun, G-Q. and Zhu, Z-L. (2007). Assessment of nitrogen pollutant sources in surface waters of Taihu lake region. Pedosphere., 17; 200–208.
Yadav, A.K., Abbassi., R., Gupta, A. and  Dadash-zadeh, M. (2013). Removal of Fluoride from aqueous solution and groundwater by wheat straw, saw dust and activated carbon of sugarcane. Ecol. Eng., 52; 211–218.