With an increase in the global pollution, there is requirement for an alternative to the fossil fuels. Non-edible vegetable oils are highly promising for producing liquid fuels like diesel. Jatropha is a potential feedstock for biodiesel, currently utilized in India and many parts of the world. The optimization of reaction conditions such as temperature, time, catalyst and molar ratio for biodiesel production is important in reactor design. However, oils have characteristics reaction properties for optimum yield. Therefore, there is the need to identify such parameters in Jatropha oil ethyl esters production. Preparation of biodiesel from Jatropha oil ethyl esters using conventional homogeneous process. Optimization of Jatropha ethyl esters using Response surface methodology is done and data so obtained are fed to the design experiment software for analysis. The Jatropha ethyl esters yield was 92.62%. Maximum production of Jatropha oil ethyl ester was achieved with the process parameters viz molar ratio 8.5, reaction time 89.67min, reaction temperature 70.1°C and catalyst.0.62wt%.