Appraisal the Protective Effects of Cymbopogon Schoenanthus Extract against Reproductive Disorders and Carcinogenic Effects of Formalin in Experimental Male Rats

Document Type : Original Research Paper

Authors

1 Food Toxicology and Contaminants Departmetn, National Research Centre, PostCode 12622, Dokki, Giza, Egypt

2 Nutrition and Food Science Departmet, National Research Centre, PostCode 12622, Dokki, Giza, Egypt

3 Molecular Biology Department, National Research Centre, PostCode 12622, Dokki, Giza, Egypt

4 Pathology Department, Faculty of Veterinary Medicine, Post Code 12211, Cairo University, Giza, Egypt

Abstract

Formaldehyde has been documented to be naturally present in many common foods. There has been a big public concern over the use of formaldehyde in the preservation of food. Also, it is commonly used as a chemical substance, usually in the life and can interact with many bio-substance in the human body. The present study target to investigate the protective effects of  Cymbopogon schoenanthus (CS) extract against the reproductive and carcinogenic effects of formaldehyde on male rats. The Albino male rats were divided into equal six groups, first group: rendered as a control group; second group: received formalin (100 mg/kg bw) and third group and forth group: were received SC extract at (50 and 100 mg) respectively; fifth group and sixth group were received formalin (100mg /kg bw) + SC extract (50mg) and formalin (100mg /kg bw)+ SC extract (100 mg) respectively. At the end of the experiment the animals were scarified and blood samples were collected for measurement all tested parameters. The results showed that the oral exposure to formaldehyde at a dose of 100 mg/kg bw resulted in significant negative effects in all tested parameters, while the CS extract at tow doses (50 and 100 mg) alone or in combination with formalin restored the negative effects to normal levels compared with the untreated group. The histopathological examination was studied on testis tissues and the histopathological pictures showed the CS extract at tow mention doses had ameliorate the adverse effects that induced by formaldehyde hazards.

Keywords

References

  1. Abdel-Wahhab, M.A., Gamil, K., El-Kady, A.A., El-Nekeety, A.A. and Naguib, K.M. (2011). Therapeutic Effects of Korean Red Ginseng Extract in Egyptian Patients with Chronic   Liver Diseases. Journal of Ginseng Research, 35(1), 69– 79.35.1.069.

     

    Abu-Serie, M.M., Habashy, N.H. and Maher, A.M. (2019). In vitro anti-nephrotoxic potential of Ammi visnaga, Petroselinum crispum, Hordeum vulgare, and Cymbopogon schoenanthus seed or leaf extracts by suppressing the necrotic mediators, oxidative stress and inflammation. BMC complementary and alternative medicine, 19(1), 149.

    Adegbegi, J.A. and Oso, K.G. (2015). The effects of Cymbopogon citratus (Lemon grass) on the antioxidant profiles wistar albino rats. Merit Research Journal of Environmental Science and Toxicology 3(4) ;051-058.

    Aebi, H. (1984). Catalase in Vitro. Methods Enzymol. 105, 121–126.

    Aitken, R.J. and Roman, S.D. (2008). Antioxidant systems and oxidative stress in the testes,” Oxid Med Cell Longev., vol. 1, no. 1, pp. 15-24.

    Bahmani, M., Zargaran, A. and Rafieian-Kopaei, (2014). Identification of medicinal plants of urmia for treatment of gastrointestinal disorders, Braz J Pharmacogn, 24(4), 468-480.

    Boudou, F., Berroukche A., Bendahmane-Salmi, M., Kandouci, B.A. and Tou, N. (2013). Ameliorative Effects of Syzygium aromaticum Essential Oil on Fertility in Male Rats Exposed to Manganese. Adv. Sex. Med., 3, 37412.

    Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 28(1): 25-30.

    Buege, J.A. and Aust, S.D. (1978). Microsomal lipid peroxidation. Methods Enzymol. 52, 302–310.

    Delfan, B., Baharvand-Ahmadi B., Bahmani, M., Mohseni N., Saki, K., Rafieian-Kopaei, M., Shahsavari, S., Naghdi, N., Taherikalani, M. and Ghafourian, S. (2015). An ethnobotanical study of medicinal plants used in treatment of kidney stones and kidney pain in Lorestan province, Iran. Journal of Chemical and Pharmaceutical Sciences, 8(4), 693-699.

    Drury, R.A., Wallington, E.A. and Cancerson, R. (1980). Carleton's Histopathological techniques. 4th ed. Oxford, London, New York: Oxford University Press.

    Elumalai, P., Krishnamoorthy, G., Selvakumar, K., Arunkumar, R., Venkataraman, P. and Arunakaran,

    1. (2009). Studies on the protective role of lycopenea gainst polychlorinated biphenyls (Aroclor1254)-induced changes in StAR protein and cytochrome P450 scc enzyme expression on Leydig cells of adult rats. Reprod. Toxicol., 27, 41– 45.

    Gibbs, P. E, Zielinski, R., Boyd, C. and Dugaiczyk, A. (1987). Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. Biochemistry, 26,1332-1343.

    Gomaa, M.N.E., Ayesh, A.M., Abdel Galil, M.M. and Naguib, K. (1997). Effect  of pressure ammoniation procedure on the detoxification   of    aflatoxins. Mycotoxin    Res., 13: 23–34.

    I.A.R.C. (2006). Formaldehyde, 2-butoxyethanol and 1-tertbutoxypropan-2-ol. IARC Monogr Eval Carcinog Risks Hum, 88: 1–478. PMID: 17366697.

    I.A.R.C. (2014). Monographs on the evaluation of carcinogenic risks to humans. Volume 100F: Chemical agents and related occupations, formaldehyde. International Agency for Research on Cancer, Lyon, France.

    Khadri, A., Neffati, M., Smiti, S., Fale, P., Lino, A.R.L., Serralheiro, M. L.M. and Araujo, M.E.M. (2010) Antioxidant, antiacetylcholinesterase and antimicrobial activities of Cymbopogon schoenanthus L. Spreng (lemon grass) from Tunisia. LWT - Food Sci Technol., 43:331–336.

    Knobil, E. (1980). The neuroendocrine control of the menstrual cycle. Rec Prog Horm Res; 36: 52-88.

    Kose, E. Sarsilmaz, M. Tas, U. Kavakli, A. Turk, Ozlem, G. and Dabak, D. (2012) Rose oil inhalation protects against formaldehyde-induced testicular damage in rats,” Andrologia, 44, 342-348.

    Lawrence, R.A. and Burk, R.F. (1979). Glutathione peroxidase activity in selenium deficient rat liver. Biochem. Biophys. Res. Commun. 71,952–958.

    Metz, B., Kersten, G. F, Hoogerhout, P, Brugghe,

    1. F, Timmermans, H. A, de jong, A, Meiring, H, ten Hove, J., Hennnink, W. E., Crommelin, D. J. and Jiskoot, W. (2004). Identification of formaldehyde- induced modifications in proteins: reaction with modle peptides. J. Biol. Chem., 279, 6235-6243.

    Makker, K., Agarwal, A. and Sharma, R. (2009). Oxidative stress and male infertility. Indian J. Med. Res., 129(4):357-67.

    Manosroi, J., Dhumtanom P. and Manosroi A. (2006). Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P388 cell lines. Cancer Lett.; 235: 114-120.

    Mardani, S., Nasri, H., Rafieian-Kopaei, M. and Hajian, S. (2015). Herbal medicine and diabetic kidney disease. J. Nephropharmacol, 2(1), 1-2.

     

    McCann, D. and Kirkish, L. (1985). Evaluation of free testosterone in serum. J Clin Immunoassay; 8: 234-6.

    Nasri, H., Bahmani, M., Shahinfard, N., Nafchi, A. M., Saberianpour, S. and Kopaei, M. R. (2015). Medicinal plants for the treatment of acne vulgaris, A review of recent evidences. Jundishapur Journal of Microbiology, 8(11), e25580.

    Ozen, O.A., Kus, M.A., Kus, I., Alkoc, O.A. and Songu, A. (2008). Protective effects of melatonin against formaldehyde-induced oxidative damage and apoptosis in rat testes: An immunohistochemical and biochemical study,” Syst. Biol. Reprod. Med., 54(4-5), 169-176.

    Peter, F., Mark, T., Naomi, H. and Graham, L. (2019). Maternal Exposure to Indoor Air Pollution and Birth Outcomes. Int. J. Environ. Res. Public Health, 16, 1364

    Popovic, M., Kaurinovic, B., Trivic, S., Mimica- Dukic, N. and Bursac, M. (2006). Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytother Res., 20(7): 531-7.

    Rasyidah, T.I. Suhana, S. Nur-Hidayah, H., Kaswandi,

    • and Noah, M. (2014). Evaluation of Antioxidant Activity of Zingiber Officinale (Ginger) on Formalin-Induced Testicular Toxicity in Rats. Journal of Medical and Bioengineering Vol. 3, No. 3, 149-153.

    Reeves, P. G., Nielsen, F. H. and Fahey, G.C. (1993). AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN- 76A rodent diet. J Nutr., 123: 1939-51.

    Said, T.M., Aziz, N., Sharma, R. K, Lewis-Jones, I., Thomas, A. J. and Agarwal, A. (2005). Novel association between sperm deformity index and oxidative stress-induced DNA damage in infertile male patients. Asian J. Androl., 7, 121–126.

    Sari, D.K., Kuwahara, S., Tsukamoto, Y., Hori, H., Kunugita, N. and Arashidani, K. (2004). Effect of prolonged exposure to low concentrations of formaldehyde on the corticotropin releasing hormone neurons in the hypothalamus and adrenocorticotropic hormone cells in the pituitary gland in female mice. Brain Res.(1):107-16.

    Seif, M.M., Ahmed-Farid, O.A. and Aboulthana, W. M. (2017).   Evaluation of the Protective Effect of Acacia senegal Extract against di-(2-ethylhexyl phthalate) Induced Hepato- and Neurotoxicity in Rats. Ann. Res. Rev. Bio,19(2): 1-17.

    Seif, M.M., Khalil, F.A., Abou-Arab, A.A.K, Abdel-Aziz, A.S., Abou-Donia. M.A. and  Mohamed, S.R. (2014). Protective Effect of Melissa officinalis L. against Malathion Toxicity and Reproductive Impairment in Male Rats International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 8(8), 886-892 .

    Sherif, S.M., Lamiaa, E., Sief, M., May, M., Sherif, R. and Abdel-Aziz, M.H.M. (2017). Protective effect of Cymbopogon schoenanthus extract against formalin hazards in rats. Res. J. Med. Plants, 11: 8- 13.

    Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdicphosphotungstic acid reagents. Am J Enol Vitic, 16(3): 144 - 158.

    Talukder, S., Hossain, M., Sarker, S. and Khan, M. (2011). Investigation into effect of crude mixture of abrus precatorius seed on hypothalamopituitary gonadal axis and development of antifertility in male rats. Bangladesh J Agr Res., 36(1): 103-9.

    Teng, S., Beard, K., Pourahmad, J., Moridani, M., Easson, E., Poon, R. and Brien, P.J. (2001). The Formaldehyde Metabolic Detoxification Enzyme Systems and Molecular Cytotoxic Mechanism in Isolated Rat Hepatocytes. Chem. Biol. Interact.; 130-132(1-3): 285-296.

    Thangam, R., Sathuvan, M., Poongodi, A., Suresh, V., Pazhanichamy, K. and Sivasubramanian, S. (2014). Activation of intrinsic apoptotic signaling pathway in cancer cellsby Cymbopogon citratus polysaccharide fractions. Carbohyd Poly,; 107: 138-150.

    Trezl, L., Hullan, L., Szarvas, T., Csiba, A. and Szende, B. (1998). Determination of endogenous formaldehyde in plants (fruits) bound to L-arginine and its relation to the folate cycle, photosynthesis and apoptosis. Acta Biol.hung., 49, 253-263.

    Uddin, M.M., Amit, S.K, Islam, S.M.R., Rahman, R., Sameera, S. and Khan, M.S. (2014). “Analyzing Time Dynamic Concentration of Formaldehyde in Fresh and Formalin Treated Fish ‘Labeo rohita’,” presented at the International Conference on Chemical Engineering, Dhaka, Bangladesh.

    Vosoughi, S., Khavanin, A., Salehnia, M., Mahabadi, H. A. and Soleimanian, A. (2012). Effects of vapor and noise on mouse testicular tissue and sperm parameters. Health Scope, vol. 1, no. 3, pp. 110-117.

    Wael, M.A., Ahmed, M.Y., Amal, M.E., Noha, E.I., Mohamed, M.S. and Amgad, K.H. (2019). Evaluation of Antioxidant Efficiency of Croton tiglium L. Seeds Extracts after Incorporating Silver Nanoparticles. Egypt. J. Chem. Vol. 62, No. 2 pp. 181 - 200.

    Wakabayashi, K. (1977). Heterogeneity of rat luteinizing hormone revealed by radioimmunoassy and electrofocusing studies. Endocrinol Jap., 24: 473-85.

    Yakubu, M.T. (2008). Effect of Cnidoscolous aconitifolius (Miller) IM Johnston leaf extract on reproductive hormones of female rats. Iran J Reprod Med., 6(3): 149-55.

    Zamcheck, N. and Martin, E.W. (1981). Sequential carcinoembryonic antigen levels in pancreatic cancer: some clinical correlations. Cancer, 47, 1620-27.

    Zhou, D., Qiu, S., Zhang, J. and Wang, H.X. (2006). Effect of Formaldehyde on Spermatogenesis and Testicular Morphology in Adult Rats. J US-China Med Sci.; 3(3):58-60.

Pollution
Volume 6, Issue 1
January 2020
Pages 211-221

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