Overall D. melanogaster Cohort Viability as A Pollution Indicator of the Atmospheric Air of Urban Landscapes

Document Type: Original Research Paper


1 Department of ecology and biomonitoring, Chernivtsi Yuriy Fedkovych National University, Chernivtsi, Ukraine

2 Department of economic geography and ecological management, Chernivtsi Yuriy Fedkovych National University, Chernivtsi, Ukraine

3 Department of communicative linguistics and translation, Chernivtsi Yuriy Fedkovych National University, Chernivtsi, Ukraine


The method of air pollution level evaluation of urban landscapes on the basis of D. melanogaster cohort analysis has been suggested. The method implies the binding to the landscape areas of the city. Within each landscape area traps and cultivators for D. melanogaster have been installed in sanitary-protective zones of various enterprises as well as on the background territory with the least level of anthropogenic load serving as the control. Based on specifically elaborated technique for field conditions, the amount of eggs, third instar larvae, pupae and imago has been calculated. Then, using the computer program ImageJ, the square under the curves of cohort survival has been determined which is considered overall cohort viability (OCVD.m). The previously mentioned indicator considers cohort survival at all stages of ontogenesis. In addition, the expressed in percentage indicator of oppression (IO OCVD.m) in relevance to the control OCVD. m affects the level of air pollution of urban landscapes by emissions of various enterprises. The relevance between these indicators is determined by a four-level scale elaborated specifically for the purpose. The method has been tested based on technogenic landscapes of Chernivtsi, Ukraine. The sensitivity of the suggested indicator for a wide range of pollutants has been proved and its ability to respond to different levels of greening of similar enterprises has been shown.


Al-Momani, F.A. and Massadeh, A.M. (2005). Effect of different heavy-metal concentrations on Drosophila melanogaster larval growth and development. Biol Trace Elem Res., 108 (1–3); 271-277.
Antonov, V.S. (2002). How the Climate of Chernivtsi Has Changed for the Last 50 Years (Chernivtsi: Misto).
Asif, N., Malik, M.F. and Chaudhry, F.N. (2018). A Review of on Environmental Pollution Bioindicators. Pollution, 4(1); 111-118. Badre, N. H., Martin, M. E. and Cooper, R. L. (2005). The physiological and behavioral effects of carbon dioxide on Drosophila melanogaster larvae. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology Special Issues, 140 (3); 363-376. Ben-Shahar, Y. (2018). The Impact of Environmental Mn Exposure on Insect Biology. Front. Genet., 9; 70-75.
Buzhdygan, O. Y., Rudenko, S. S., Kazanci, C. and Patten, B. C. (2016). Effect of invasive black locust (Robinia pseudoacacia L.) on Nitrogen cycle in floodplain ecosystem. Ecological Modelling, 319; 170-177.
Buzhdygan, O. Y., Rudenko, S. S., Patten, B. C. and Kostyshyn, S. S. (2014). Food-web topology of Ukrainian mountain grasslands: Comparative properties and relations to ecosystem parameters. Ecological Modeling, 293; 128-138.
Cargando, D. C. and Real, M. S. B. (2013). Effects of exposure to sulfur dioxide on the histology of fruit fly (Drosophila melanogaster) gonads and fat bodies. (Bachelor thesis of BS Biology students).
Castañeda, P.L., Muñoz, G.L.E., Durán, D.A., Heres, P.M.E. and Dueñas, G.I.E. (2001). LD50 in Drosophila melanogaster fed on lead nitrate and lead acetate. Dros. Inf. Serv., 84; 44-48.
Cherniak, L.M. and Radomska, M.M. (2012). Modern Methods of Decreasing Negative Impact of Gas Stations on Environment. Science-Based Technologies, 3 (15); 44-47.
Cohn, J., Widzowski, D.V. and Cory-Slechta, D.A. (1992). Lead retards development of Drosophila melanogaster. Comp Biochem Physiol Part C: Comparative Pharmacology.102(1); 45-49.
Colinet, H. and Renault, D. (2012). Metabolic effects of CO2 anaesthesia in Drosophila melanogaster. Biology Letters, 8(6); 1050-1054.
de Santana, S.L., Verçosa, C.J., de Araújo, C. Í.F, de Amorim, É.M, da Silva, A.S, da Rocha Bastos, T.M, da Silva Neto, L.J., dos Santos, T.O., de França, E.J. and Rohde, C. (2018). Drosophila melanogaster as model organism for monitoring and analyzing genotoxicity associated with city air pollution. Environ Sci Pollut Res Int., 25(32); 32409-32417.
Ecological Passport of Chernivtsi Region (2018). (Chernivtsi: Department of Ecology and Natural Resources of Chernivtsi Regional State Administration).
Rudenko, S.S., et al.
Enikolopov, G., Banerji, J. and Kuzin, B. (1999). Nitric oxide and Drosophila development. Cell Death Differ., 6(10); 956-963.
Eom, H.-J., Yuedan, L., Kwak, G-S., Heo, M., Song, K. S., Chung, Y. D., Chon, T.-S. and Choi, J. (2017). Inhalation toxicity of indoor air pollutants in Drosophila melanogaster using integrated transcriptomics and computational behavior analyses. Scientific Reports, 7 (46473). Fly Facility: Fly food (2018). (University of Sheffield: The Bateson Centre).
Foley, E. and O’Farrell, P.H. (2003). Nitric oxide contributes to induction of innate immune responses to gram negative bacteria in Drosophila. Genes Dev., 17 (1); 115-125.
GadzaƂa-Kopciuch, R., Berecka, B., Bartoszewicz, J. and Buszewski, B. (2004). Some Considerations About Bioindicators in Environmental Monitoring. Polish Journal of Environmental Studies, 13 (5); 453-462.
Gerdnes, R.A., Smith, J.D. and Applegate, H.G. (1971). The effects of atmospheric hydrogen fluoride upon Drosophila melanogaster. II. Fecundity, hatchability and fertility. Atmos Environ., 5(3); 117-122.
Ginevan, M.E. and Lane, D.D. (1978). Effects of sulfur dioxide in air on the fruit fly, Drosophila melanogaster. Environmental Science & Technology, 12 (7); 828-831.
Hirsch, H.V.B., Lnenicka, G., Possidente, D., Possidente, B., Garfinkel, M. D., Wang, L., Lu, X. and Ruden, D.M. (2012). Drosophila melanogaster as a model for lead neurotoxicology and toxicogenomics research. Front. Genet., 3 (Article 68); 1-7. Holt, E. A. and Miller, S. W. (2010). Bioindicators: Using Organisms to Measure Environmental Impacts. Nature Education Knowledge, 3 (10); 8-15.
Hutsuliak, V.M. (2006). Chernivtsi Landscapes: monograph (Chernivtsi: Ruta).
Inamdar, A.A., Masurekar, P. and Bennett, J.W. (2010). Neurotoxicity of fungal volatile organic compounds in Drosophila melanogaster. Toxicol Sci., 117(2); 418-426.
Inamdar, A.A., Zaman, T., Morath, S.U., Pu, D.C. and Bennett, J.W. (2014). Drosophila melanogaster as a model to characterize fungal volatile organic compounds. Environ Toxicol., 29(7); 829-836.
Jaszczak, J.S., Wolpe, J.B., Dao, A.Q. and Halme, A. (2015). Nitric Oxide Synthase Regulates Growth Coordination During Drosophila melanogaster Imaginal Disc Regeneration. Genetics, 200; 1219-1228.
Kennedy, G.L, Arnold, D., Keplinger, M.L. and Calandra, J.C. (1971). Mutagenic and teratogenic studies with lead acetate and tetraethyl lead. Toxicol Appl Pharmacol, 19; 370-375.
Kobevko, T. “ROSMA” Company: The Way to Stability. State Information Bulletin on Privatization, 3(270); 18.
Koniuhov, A.L. (2012). Manual of the Use of Program Complex ImageJ for Image Processing (Tomsk:TUSUR).
Kucera, J. (1993). Biological monitors of air pollution. (Praga: Czech Ecological Institute, NAA Laboratory).
Li, Y. and He, R. (2016). The Effects of Formaldehyde on Life Span and Stress Resistance in Drosophila melanogaster. Progress in Biochemistry and Biophysics, 43(4); 419-428.
Li, Y. and He, R. (2017). Formaldehyde Affecting Lifespan and Stress Resistance in Drosophila. (In He, R. (Eds.) Formaldehyde and Cognition. Chapter 11 (pp. 209-219). Beijing: Springer Science+Business Media B.V.).
Liang, X. H., Locantore, A., Martinez, M. and Kaur, K. (2018). The Developmental effects of Formaldehyde on Drosophila melanogaster. The Journal of Biological Sciences, 4; 10-13.
MacGregor, M.Ch., Trivino, V., Luu, B., Moreno, A., Kovar, E., Arredondo, D., Rosell, R. and Ledesma, E. (2018, Apr 20). Correlation between toluene exposure and toxicity effects of toluene on Drosophila melanogaster fecundity and offspring survivability. The FASEB Journal, 32(1_supplement), Abstract Number: 692.1 (Abstract of the Experimental Biology 2018 meeting).
Markert, B.A., Breure, A.M. and Zechmeister, H.G. (2003). Bioindicators and Biomonitors: Principles, Concepts and Applications. Trace Metals and other Contaminants in the Environment, 6; 15-25.
Mathew, B.B. and Krishnamurthy, N.B. (2018). Assessment of Lead Toxicity Using Drosophila melanogaster as a Model. India J Clin Toxicol, 8(2); 380-386.
Meyer, C. (2013a, September 8-12). The Greening of the Concrete Industry (The 2013 World Congress on Advances in Structural Engineering and Mechanice (ASEM13), Jeju, Korea).
Meyer, C. (2013b). Concrete as a Green Building Material (New York: Columbia University).
Mit’, N., Amirgalieva, A., Begmanova, M., Tolebaeva, A. and Djansugurova, L. (2014). The alterations in Drosophila melanogaster lifespan due to nitric oxide synthase donors and inhibitors influence. Rezyumethe alterations in Drosophila melanogaster lifespan due to nitric oxide synthase
Pollution, 6(2): 317-333, Spring 2020
Pollution is licensed under a "Creative Commons Attribution 4.0 International (CC-BY 4.0)"
donors and inhibitors influence. Visnyk of the Lviv University. Series Biology, 66; 72-78.
Morley, E. J., Hirsch, H. V. B., Hollocher, K. and Lnenicka, G. A. (2003). Effects of Chronic Lead Exposure on the Neuromuscular Junction in Drosophila Larvae. Neurotoxicology, 24 (1); 35-41.
Nassar, R. (1979). Genetics of resistance to tetraethyllead. Aust J Biol Sci., 32(1); 127-132.
Nylander, P. O., Olofsson, H., Rasmuson, B. and Svahlin, H. (1978). Mutagenic effects of petrol in Drosophila melanogaster I. Effects of benzene and 1,2-dichloroethane. Mutat Res., 57; 163-167.
Peterson, E. K., Wilson, D.T., Possidente, B., McDaniel, Ph., Morley, E.J., Possidente, D., Hollocher, K. H., Ruden, D.M., and Hirsch, H.V.B. (2017). Accumulation, elimination, sequestration, and genetic variation of lead (Pb2+) loads within and between generations of Drosophila melanogaster. Chemospere, 181; 368-375.
Prokopenko, O. (Ed.) (2018). Statistical yearbook environment of Ukraine 2017. (Kyiv: State Statistics Service of Ukraine).
Rabinovich, D., Yaniv, S.P., Alyagor, I. and Schuldiner, O. (2016). Nitric Oxide as a Switching Mechanism between Axon Degeneration and Regrowth during Developmental Remodeling. Cell., 164 (1-2); 170-182.
Rand, M.D., Montgomery, S.L., Prince, L. and Vorojeikina, D. (2014). Developmental Toxicity Assays Using the Drosophila Model. Current Protocols in Toxicology, 59 (20); 1.12.1-1.12.20.
Rudenko, S.S. and Leheta, U.V. (2007). Patent on Useful Model 20908 UA MPK: G01N 33/18, G01N 33/24 The way of biotesting of ecological state of technogenically transformed territories; claimer Chernivtsi Yuriy Fedkovych National University. – Claimed 04.09.2006; publ.. 15.02.2007, Patent Base of Ukraine: Bull. No 2.
Rudenko, S.S. and Leheta, U.V. (2011). Population Ecology with Drosophila Practicum: Cohot Analysis. (Chernivtsi: Chernivtsi National University).
Rudenko, S.S., Leheta, U.V. and Strashnyuk, V.Y. (2019a). Using ImageJ for evaluation of the effect of technogenic landscapes on viability of Drosophila melanogaster cohorts. Slovak international scientific journal, 25 (2); 18-24.
Rudenko, S.S., Tom`yuk, A.V. and Kostyshyn, S.S. (2019b). Comparative analysis of the effectiveness of a posteriori tests in cohort analysis (by example D. melanogaster), Science and Education a New Dimension. Natural and Technical Sciences, VII(24), Issue: 200; 39-44.
Safaee, S., Fereidoni, M., Mahdavi-Shahri, N., Haddad, F. and Mirshamsi, O. (2014). Effects of lead on the development of Drosophila melanogaster. Periodicum Biologorum, 116 (3); 259-265.
Salthammer, T. (2015). The Formaldehyde Dilemma. Int. J. Hyg. Environ. Health., 218; 433-436.
Salthammer, T., Mentese, S. and Marutzky, R. (2010). Formaldehyde in the Indoor Environment. Chem. Rev.,110; 2536-2572.
Schuler, R.L. and Niemeier, R.W. (1981). A study of diesel emissions on Drosophila. Environment International, 5 (4-6); 431-434.
Statistical Data on Automobile Transport (2018). (Kyiv: Ministry of Infrastructure of Ukraine). Ternes, A. P., Zemolin, A. P., Da Cruz, L. C., Da Silva, G. F., Saidelles, A. P. and De Paula, M. T. (2014). Drosophila melanogaster – an embryonic model for studying behavioral and biochemical effects of manganese exposure. EXCLI J., 13; 1239-1253.
Timonina, M.B. (Ed.) (2018). The Population of Ukraine (Kyiv: State Statistics Service of Ukraine). Trivino, V., MacGregor, M., Nguyen, T., Nunez, B., Lodhra, Z., Castillo, L., Brenda, L., Rosell, R. and Ledesma, E. (2017, Apr 01). Determining the correlation between Drosophila melanogaster toluene exposure and the resulting toxicity effects on fly survival and fecundity. The FASEB Journal, 31(1_supplement), Abstract Number:609.16 (Abstract of the Experimental Biology 2017 meeting).
Tsaryk, J.V. (2011). Population Ecology: Gains and Perspectives. Biol.Stud., 5 (3); 171-182.
Vache, C., Camares, O., de Graeve, F., Dastugue, B., Meiniel, A., Vaury, C., Pellier, S., Leoz-Garziandia, E. and Bamdad, M. (2006). Drosophila melanogaster p-glycoprotein: a membrane detoxification system toward polycyclic aromatic hydrocarbon pollutants. Environmental Toxicology and Chemistry, 25 (2); 572-580.
Weather Archive of Chernivtsi (July-August, 2018). Access mode: Rp5.ua/ Archive_of Chernivtsi_ weather_(airport).
Zillioux, E.J. (2009). Environmental Bioindicators (EBI) to Environmental Indicators (EI) and other Developments: the Evolution of a Journal. Environmental Bioindicators, 4 (4); 283-285.