Potential Application of Synchronous Fluorescence Spectroscopy to Identification of PAHs in Airborne PM2.5

Document Type : Original Research Paper


1 Department of Physics, Amity Institute of Applied Sciences, Amity University, Sector 125, Noida - 201 313, India

2 Tezpur University, Tezpur - 784028, Assam, India

3 Department of Physics, Jamia Millia Islamia, New Delhi - 110025, India


A simple and rapid method for the highly sensitive determination of polycyclic aromatic hydrocarbons (PAHs) from airborne fine particulate matter (PM2.5) in an urban environment of Delhi was developed. The target compounds were 10 of the 16 United States Environmental Protection Agency (US-EPA) priority PAHs: fluoranthene, pyrene, chrysene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo[a]pyrene, dibenzo(ah)anthracene, benzo(ghi)perylene, indeno(1,2,3-cd)pyrene. For collecting the samples, the following two locations in Delhi (India) were chosen: ITO and Okhla Industrial Area.  Two sets of samples at these locations of were collected for the purpose of investigation. The fine particulate matter samples were collected on glass fiber filter papers for 24h, from which the PAHs were extracted using dichloromethane (DCM) and hexane using ultrasonication method. Comparison of the characteristic emission of spectra of PAHs with standard spectra indicated the degree of condensation of aromatic compounds present in the investigated mixtures. However, this identification could be more effective with the use of the respective values of Δλ parameter for each particular component of the mixture. It has been found that the concentration of the PAHs is maximum during the winter season and minimum during the summer and monsoon seasons at both the locations. 


Apicella, B., Ciajolo, A. and  Tregrossi, A. (2004). Fluorescence Spectroscopy of Complex Aromatic Mixtures. Analytical Chemistry, 76, 2138–2143.
Boeuf, B., Fritsch, O. and Martin-Ortega, J. (2016). Undermining European Environmental Policy Goals? The EU water framework directive and the politics of exceptions. Water, 8 (9) 388.
Crimmins, B.S., Dickerson, R.R., Doddridge, B.G. and Baker, J.E. (2004). Particulate polycyclic aromatic hydrocarbons in the Atlantic and Indian Ocean atmospheres during the Indian Ocean Experiment and Aerosols 99: Continental sources to the marine atmosphere. Journal of Geophysical Research Atmospheres: JGR, 109, D05308.
Delhi Statistical Handbook (2016). Directorate of Economics and Statistics. India Environmental Portal. Retrieved December 20, 2021, from
Ding, X., Wang, X., Xie, Z., Xiang, C., Mai, B. and Sun, L. (2007). Atmospheric polycyclic aromatic hydrocarbons observed over the North Pacific Ocean and the Arctic area: Spatial distribution and source identification. Atmospheric Environment, 41, 2061–2072.
Eiroa, A.A., Blanco, E.V., Mahía P.L., Lorenzo, S.M. and Rodríguez, D.P. (2000). Resolution of benzo(a) pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Analyst, 125, 1321-1326.
Esen, F., Tasdemir, Y. and Cindoruk, S. S. (2010). Dry deposition, concentration and gas/ particle partitioning of atmospheric carbazole. Atmospheric Research, 95, 379–385.
Friedman, C. L., Zhang, Y. and Selin, N. E. (2013). Climate change and emissions impacts on atmospheric PAH transport to the Arctic. Environmental Science & Technology, 48, 429–437.
Gargiulo, V., Apicella, B., Alfè, M., Russo C., Stanzione, F., Tregrossi; A., Amoresano, A., Millan M., & Ciajolo, A. (2015). Structural Characterization of Large Polycyclic Aromatic Hydrocarbons. Part 1: The Case of Coal Tar Pitch and Naphthalene-Derived Pitch. Energy & Fuels, 29, 5714-5722.
Gargiulo, V., Apicella, B., Stanzione, F., Tregrossi, A., Millan, M., Ciajolo, A., &  Russo, C. (2016).Structural Characterization of Large Polycyclic Aromatic Hydrocarbons. Part 2: Solvent-Separated Fractions of Coal Tar Pitch and Naphthalene-Derived Pitch. Energy Fuels, 30, 2574–2583.
Hanedar, A., Alp, K., Kaynak, B. and Avşar, E. (2014). Toxicity evaluation and source apportionment of polycyclic aromatic hydrocarbons (PAHs) at three stations in Istanbul, Turkey. Science of the Total Environment, 488, 437–446.
Hao, Y., Gao, C., Deng, S., Yuan, M., Song, W., Lu, Z. and Qiu, Z. (2019). Chemical characterization of PM2.5 emitted from motor vehicles powered by diesel, gasoline, natural gas and methanol fuel. Sci. Total Environ. 674: 128–139.
Hazarika, N., Das, A., Kamal, V., Anwar, K., Srivastava, A. and Jain, V. K. (2019). Particle phase PAHs in the atmosphere of Delhi-NCR: With spatial distribution, source characterization and risk approximation. Atmospheric Environment, 200, 29-342.
He, X., Pang, Y., Song, X., Chen, B., Feng, Z. and Ma, Y. (2014). Distribution, sources and ecological risk assessment of PAHs in surface sediments from Guan River Estuary, China. Marine Pollution Bulletin, 80, 52-58.
Jamhari, A.A., Sahani, M., Latif, M.T., Chan, K.M., Tan, H.S., Khan, M.F. and Tahir, N.M. (2014) Concentration and source identification of polycyclic aromatic hydrocarbons (PAHs) in PM10 of urban, industrial and semi-urban areas in Malaysia. Atmospheric Environment, 86, 16–27.
Krupadam, R.J., Bhagat, B. and Khan, M.S. (2010). Highly sensitive determination of polycyclic aromatic hydrocarbons in ambient air dust by gas chromatography-mass spectrometry after molecularly imprinted polymer extraction. Analytical and Bioanalytical Chemistry, 397(7)3097-3106.
Kumar, P., Gulia, S., Harrison, R.M., & Khare, M. (2017). The influence of odd–even car trial on fine and coarse particles in Delhi. Environmental Pollution.225, 20–30.
Kumar, A., Ambade, B., Sankar, T. K., Sethi, S. S. and Kurwadkar, S. (2020). Source identification and health risk assessment of atmospheric PM2.5 bound polycyclic aromatic hydrocarbons in Jamshedpur, India. Sustainable Cities and Society, 52, 101801.
Lage-Yusty, M.A., Lopez-Gonzalez, J. and Simal-Lozano, J. (2005). Resolution of 13 polycyclic aromatic hydrocarbons by constant wavelength synchronous spectrofluorometry. Analytical Sciences 21, 1203.
Li, C., Wu, F., & Caikandiyoti, H.R. (1994). UV-Fluorescence Spectroscopy of Coal Pyrolysis Tars. Energy Fuels, 8, 1039-1048.
Lin, Y.C., Li, Y.C., Amesho, K.T.T., Chou, F.C. and Cheng, P.C. (2019). Characterization and quantification of PM2.5 emissions and PAHs concentration in PM2.5 from the exhausts of diesel vehicles with various accumulated mileages. Sci. Total Environ. 660: 188–198.
Liu, L.B., Liu, Y., Lin, J.M., Tang. N., Hayakawa, K., & Maeda, T. (2007). Development of Analytical Methods for Polycyclic Aromatic Hydrocarbons (PAHs) in Airborne Particulates: A Review. Journal of Environmental Sciences, 19, 1-11.
Liu, Di., Xu, Y., Chaemfa, C., Tian, C., Li, J., Luo, C. and Zhang, G. (2014). Concentrations, seasonal variations, and outflow of atmospheric polycyclic aromatic hydrocarbons (PAHs) at Ningbo site, Eastern China. Atmospheric Pollution Research 5, 203–209.
Lloyd, J.B.F. (1971).  Synchronized Excitation of Fluorescence Emission Spectra. Nature, 231, 64-65.
Lloyd, J.B.F. (1974). Partly quenched, synchronously excited fluorescence emission spectra in the characterisation of complex mixtures. Analyst. 99, 729.
Lopez de Alda Villaizan, M. J., Alvarez Pineiro, M. E., Garcia-Falcon, M.  S., Lage-Yusty, M.A., & Simal-Lozano, J. (1994). Hexane as solvent for determination of PAHs in spectrofluorometry. Analusis, 22, 495-498.
Lopez de Alda, M. J., Garcia-Falcon, S., Lage-Yusty, M.A. and Simal-Lozano, J. (1995). Synchronous Spectrofluorimetric Determination of Total Amounts of the Six Polycyclic Aromatic Hydrocarbons Officially Designated as Indicators of Drinking Water Quality. J. Assoc. Off. Analytical Chemistry, 78 (2), 402 - 406.
Matuszewska, A. and Czaja, M. (2000). The use of synchronous luminescence spectroscopy in qualitative analysis of aromatic fraction of hard coal thermolysis products. Talanta, 52, 457–464.
Matuszewska, A. and Czaja, M. (2002). Aromatic compounds in molecular phase of Baltic amber—synchronous luminescence analysis. Talanta, 56, 1049–1059.
Patra, D. and Mishra, A.K. (2001). Investigation on simultaneous analysis of multicomponent polycyclic aromatic hydrocarbon mixtures in water samples: a simple synchronous fluorimetric method. Talanta, 55, 143–153.
Poster, D.L., Schantz, M.M., Sander, L.C. and Wise, S.A. (2006). Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) in Environmental Samples: A Critical Review of Gas Chromatographic (GC) Methods. Analytical and Bioanalytical Chemistry, 386, 859–881.
Sharma, H., Jain, V. K. and Khan, Z. H. (2007). Characterization and source identification of polycyclic aromatic hydrocarbons (PAHs) in the urban environment of Delhi. Chemosphere, 66, 302–310.
Sharma, H., Jain, V.K. and Khan, Z.H. (2007). Identification of polycyclic aromatic hydrocarbons (PAHs) in suspended particulate matter by synchronous fluorescence spectroscopic technique.  Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 68, 43–49.
Sharma, H., Jain, V.K. and Khan, Z.H. (2008). Atmospheric polycyclic aromatic hydrocarbons (PAHs) in the urban air of Delhi during 2003. Environmental Monitoring and Assessment, 147, 43–55.
Sharma, H., Jain, V.K. and Khan, Z.H., (2013). Use of constant wavelength synchronous spectrofluorimetry for identification of polycyclic aromatic hydrocarbons in air particulate samples. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 108, 268–273.
Shahpoury, P., Kitanovski, Z. and Lammel, G. J. A. C. (2018). Snow scavenging and phase partitioning of nitrated and oxygenated aromatic hydrocarbons in polluted and remote environments in central Europe and the European Arctic, Atmos. Chem. Phys., 18, 13495- 13510.
Sisovic, A., Belsic, A., Sega, K. and Vadjic, V. (2008). PAH mass concentrations measured in PM10 particle fraction. Environment International, 34, 580–584.
Sharma, M. and Dikshit, O. (2016). Report on Comprehensive study on air pollution and greenhouse gases (GHGs) in Delhi & DPCC: Dept. of Env. Govt. of NCT. Retrieved December 20, 2021, from
Statistical Abstract of Delhi, Directorate of economics and statistics. Government of National Capital Territory of Delhi (2016).  www.delhi.gov.in.
United States Environmental Protection Agency (1999). Compendium of Methods for the Determination of Toxic Organic Compounds in Ambient Air, Second Edition. EPA/625/R-96/010b. Retrieved January 3, 2022, from https://www3.epa.gov/ttnamti1/files/ambient/airtox/tocomp99.pdf.
Venkataraman, C., Lyons, J. M. and Friedlander, S. K. (1994). Size distributions of polycyclic aromatic hydrocarbons and elemental carbon. 1. sampling, measurement methods, and source characterization. Environmental Science and Technology, 28, 555–562.
Vo-Dinh T. (1978). Multicomponent analysis by synchronous luminescence spectrometry. Analytical Chemistry, 50, 396-401.
Wang, W., Huang, M.J., Chan, C.Y., Cheung, K.C. and Wong, M.H. (2013). Risk assessment of non-dietary exposure to polycyclic aromatic hydrocarbons (PAHs) via house PM2.5, TSP and dust and the implications from human hair. Atmospheric Environment, 73, 204 –213.
Wang, B., Lau, Y.S., Huang, Y., Organ, B., Chuang, H.C., Ho, S.S.H., Qu, L., Lee, S.C. and Ho, K.F. (2021). Chemical and toxicological characterization of particulate emissions from diesel vehicles. J. Hazard. Mater. 405, 124613.
Wu, D., Zhang, F., Lou, W., Li, D. and Chen, J. (2017). Chemical characterization and toxicity assessment of fine particulate matters emitted from the combustion of petrol and diesel fuels. Sci. Total Environ. 605–606: 172–179.
Xue, L., Lang, Y., Liu, A. and Liu, J. (2010). Application of CMB model for source apportionment of polycyclic aromatic hydrocarbons (PAHs) in coastal surface sediments from Rizhao offshore area, China. Environmental Monitoring and Assessment, 163, 57-65.
Yang, H.H., Dhital, N.B., Wang, L.C., Hsieh, Y.S., Lee, K.T., Hsu, Y.T. and Huang, S.C. (2019). Chemical characterization of fine particulate matter in gasoline and diesel vehicle exhaust. Aerosol Air Qual. Res. 19, 1439−1449.