Oxidative Stress Induction in Cassava Plant (Manihot Esculenta Crantz) Grown on Soil Contaminated with Diesel

Document Type : Original Research Paper

Authors

1 Department of Biochemistry, University of Lagos, PMB 12003 Lagos, Nigeria

2 Environmental Biotechnology Laboratory, Department of Biochemistry, Redeemer’s University, PMB 230 Ede, Osun State, Nigeria African Centre of Excellence for Water and Environmental Research (ACEWATER), Redeemer’s University, P.M.B 230, Ede, 232101, Osun State, Nigeria

Abstract

The induction of oxidative stress in plants grown on crude oil-contaminated soils was investigated using a diesel contaminated soils model. Twelve cassava stems were grown in four garden pots containing different amounts of diesel oil as contaminants: 150 ppm, 300 ppm, 600 ppm and control (0 ppm). The growth of the plants was monitored for 12 weeks, after which chlorophyll contents, total proteins, lipid peroxidation and activities of catalase, glutathione, and superoxide dismutase (antioxidant enzymes) were determined from the leaves. Significant decreases (p<0.05) were observed in the antioxidant enzymes (67-86%), total proteins (79%) and total chlorophyll content (67%) in the cassava grown on diesel contaminated soil (600 ppm) compared to the control. Consequently, there were significant increase (p<0.05) in the leaf ratio and malondialdehyde (a marker for lipid peroxidation) 0.1909 ± 04 and 1.77 ± 0.34, when compared to the control 0.1530 ± 08 sq.cm/g and 0.10±0.01 µmol/mg protein respectively. It was thus concluded that stunted growth of plants and their death in diesel or crude oil contaminated soil could be traced to oxidative stress.

Keywords

References

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Pollution
Volume 8, Issue 2
April 2022
Pages 671-679

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