Optimization of Sulphate-Reducing Bacteria for Treatment of Heavy Metals-Containing Laboratory Wastewater on Anaerobic Reactor

Document Type : Original Research Paper


1 Environmental Chemistry Laboratory, Faculty of Math and Sciences, Udayana University, Jimbaran, Badung-Bali, Indonesia

2 Environmental Engineering Study Programme, Faculty of Engineering, Udayana University, Jimbaran, Badung-Bali, Indonesia

3 Chemistry Study Program, Faculty of Math and Sciences, Udayana University, Jimbaran, Badung-Bali, Indonesia


Laboratory wastewater is categorized as hazardous waste that should not be released into the environment since it poses a serious threat to environmental safety. In the present study, the use of Sulphate-Reducing Bacteria (SRB) colonies in an anaerobic reactor to treat heavy metals-containing laboratory wastewater was examined. SRB was initially cultivated with the treatment of fermented compost and Postgate's medium before being attached to the laboratory-size anaerobic reactor to treat laboratory waste containing heavy metal. Within the 15 days of initial incubation under the room temperature of 28 °C, we discovered that SRB optimally grew on the medium with the composition of 5% Postgate B solution, 30% fermented compost liquid, and 5% active suspension liquid, with a total population of cell colonies was 1.2 x 105 CFU/ml. After SRB colonies from the most optimum medium were affixed to the reactor, the reactor attained 89% of lead (Pb) removal, 69.78% of iron (Fe) removal, and 48.93% of copper (Cu) removal for 15 days treatment periods. On the 21st days of treatment time, the removal efficiency increased significantly to 91.57%, 78.09%, and 83.56% of Pb, Fe, and Cu removed, respectively.


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