Studying Some Blood Parameters of Otolithes ruber (Schneider, 1801) in Cold and Warm Seasons as an Indicator of Pollution in Musa Creek

Document Type : Original Research Paper


Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran


The present study attempts to investigate some blood parameters of Otolithes ruber during different seasons in terms of both temperature and pollution. For so doing it uses 10 specimens, for each station and season, collected from 5 polluted stations, including Petrochemical, Ghanam, Zangi, Douragh, Patil, and Sajafi as the control group, away from pollution in Musa Creek. The fish are anesthetized with 1ml of clove extract per liter. Their blood samples are taken immediately from the caudal vein, using a heparinized syringe. Afterwards, the serum is separated in a centrifuge with a speed of 6000 rpm for 2 minutes. The desired factors are measured by the Mindray BS200 auto-analyzer and the total protein level, by Bradford's usual laboratory methods. Results show that AST, ALT, ALP, Glucose, and Triglycerides have increased in more polluted stations (P≤0.05). In sheer contrast, total protein and Albumin have decreased as pollution grows (P≥0.05). According to this study, environmental water pollution of the fish has a large impact on the concentration of measured blood parameters, whereas the influence of seasonal changes on most of them is low.


Abdi, R., Sheibani, M.T., Adibmoradi, M. and Sharifpour, I. (2007). Histological study of liver and pancreas in adult Otolithes ruber in Bushehr, Iran. Iran. J. Fish. Sci., 15(4); 87-96.
Adedeji, O.B., Adeyemo, O.K. and Agbede, S.A. (2009). Effects of diazinon on blood parameters in the African catfish (Clarias gariepinus). Afr. J. Biotechnol., 8, 3940-3946.
Alishahi, M., Mesbah, M. and Ghorbanpoor, M. (2011). Toxicity study of silver nanoparticles in four fish species. Iran. J. Vet. Res., 7, 36-41.
Aluru, N. and Vijayan, M. M. (2009). Stress transcriptomics in fish: Arole for genomic Cortisol signaling. Comp. Endocrinol., 164, 142-150.
Azarmanesh, H., Nabavi, S. M. B., Abdi, R. and Archangi, B. (2018). Effects of the Sediment Grain Size on Metabolic Reaction of Callista umbonella in Oxidative Stress Caused by Hydrocarbon Pollution in the Coast of Assaluyeh (North of the Persian Gulf). J. Oceanogr., 9(35); 11-20.
Banaee, M., Sureda, A., Zohiery, F., Hagi, B.N. and Garanzini, D.S. (2014). Alterations in biochemical parameters of the freshwater fish, Alburnus mossulensis, exposed to sublethal concentrations of Fenpropathrin. Int. J. Aquat. Biol., 2, 58-68.
Banaee, M., Sureda, A., Mirvaghefi, A.R. and Ahmadi, K. (2011). Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pestic. Biochem. Physiol., 99, 1-6.
Basir, Z. and Abdi, R. (2016). Histological study of WBC and hematological indices of spotted catshark Chiloscyllium punctatum in Persian Gulf during the cold season. J Mar Sci Technol., 14(4); 15-21.
Bastami, A.A. (2014). Concentration of Some Heavy Metal in Sediment and Fish Species from Persian Gulf. World Appl. Sci. J., 31(9); 1666-1668.
Bilberg, K., Hovgaard, M.B., Besenbacher, F. and Baatrup, E. (2012). In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio ). J. Toxicol., 1-9.
Pollution 2021, 7(2): 333-340 339
Daphedar, A. and Taranath T.C. (2018). Characterization and cytotoxic effect of biogenic silver nanoparticles on mitotic chromosomes of Drimia polyantha (Blatt. & McCann) Stearn. Toxicol. Rep., 5, 910-918.
Davis, A.K., Maney, D.L. and Maerz, J.C. (2008). The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Funct. Ecol., 22, 760-772.
Dehghan Madiseh, S., Savary, A., Parham, H. and Sabzalizadeh, S. (2009). Determination of the level of contamination in Khuzestan coastal waters (Northern Persian Gulf) by using an ecological risk index. Environ. Monit. Assess., 159, 521-530
Dogan, D. and Can, C. (2011). Hematological, biochemical, and behavioral responses of Oncorhynchus mykiss to dimethoate. Fish Physiol Biochem., 37(4); 951-958.
Eskandari, Gh., Koochaknejad, E., Hashemi, S.A., 2019. Estimation of Otolithes ruberstock with virtual population analysis in the Northwestarea of the Persian Gulf. Iran. J. Fish. Sci., 18(2); 296-306.
Hedayati, A., Safahieh A., Savari, A. and Ghofleh Marammazi, J. (2010). Assessment of aminotransferase enzymes in Yellowfin sea bream (Acanthopagrus latus) under experimental condition as biomarkers of mercury pollution. World J. Fish Mar. Science, 2(3); 186–192.
Jahan, S., Yusoff, I.B., Alias, Y.B. and Abu Bakar A.F.B. (2017). Reviews of the toxicity behavior of five potential engineered nanomaterials (ENMs) into the aquatic ecosystem. Toxicol. Rep., 4, 211-220.
Johari, S.A., Kalbassi, M.R., Soltani, M. and Yu, I.J. (2013). Toxicity comparison of colloidal silver nanoparticles in various life stages of rainbow trout (Oncorhynchus mykiss). Iran. J. Fish. Sci., 12, 76 -95.
Kavitha, C., Ramesh, M., Kumaran, S.S. and Lakshmi, S.A. (2012). Toxicity of Moringa oleifera seed extract on some hematological and biochemical profiles in a freshwater fish, Cyprinus carpio. Exp. Toxicol. Pathol., 64, 681-687.
Kazemi, S.H., Paighambari, S.Y., Daliri, M. and Abaspour Naderi, R. (2013). Length-weight and length-length relationships, condition factors and optimal length of some fish species from the Persian Gulf and Oman Sea. Int. J. Aquat. Biol., 1(4); 167-174.
Koohkan, O., Abdi, R., Zorriehzahra, S. J., Movahedinia, A. and Sharifpoor, I. (2014). Acute mortality of Liza klunzingeri in Persian Gulf and Oman Sea associated with nervous necrosis. Comp. Clin. Path., 23(2); 367-370.
Korkmaz, N., Cengiz, E., Unlu, E., Uysal, E. and Yanar, M. (2009). Cypermethrin-induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus), and the protective and recuperative effect of ascorbic acid. Environ. Toxicol. Pharmacol., 28, 198-205.
Krasno, A., Timmerhaus, G., Afanasyev, S., Takle, H. and Jorgensen, S.M. (2013). Induced erythropoiesis during acute anemia in Atlantic salmon: A transcriptomic survey. Gen. Comp. Endocrinol., 192(10); 181-190.
Kumar, N., Antony Jesu Prabhu, P., Pal, A., Remya, S., Aklakur, M., Rana, R., Gupta, S., Raman, R. and Jadhao, S. (2011). Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pestic. Biochem. Physiol., 99, 45-52.
Mahmoud, U.M., Mekkawy, I.A.A., Naguib, M. and Sayed A.H. (2019). Silver nanoparticle-induced nephrotoxicity in Clarias gariepinus: physio-histological biomarkers. Fish Physiol. Biochem., 45(6); 1895-1905.
Mekkawy, I.A., Mahmoud, U.M., Hana, M.N. and Sayed A.H. (2019). Cytotoxic and hemotoxic effects of silver nanoparticles on the African Catfish, Clarias gariepinus (Burchell, 1822) Ecotoxicol. Environ. Saf., 171, 438-446.
Murugesan, A. G., Ramathilaga, A. and Haniffa, M. A. (2013). Haematotoxicity of integrated textile mill effluent to an air-breathing fish Heteropneustes fossilis (Bloch). Bull. Environ. Contam. Toxicol., 90(5); 596-600.
Patil, V.K. and David M. (2008). Behaviour and respiratory dysfunction as an index of malathion toxicity in the freshwater fish, Labeo rohita (Hamilton). Turkish. J. Fish. Aquat. Sci., 8, 233-237.
340 Ghasemi et al.
Safahieh, A., Monikh, F. A., Savari, A. and Doraghi, A. (2011). Heavy metals concentration in Mullet Fish, Liza abu from petrochemical waste receiving creeks, Musa Estuary (Persian Gulf). J. Environ. Prot. Sci., 2(9); 1218-1223.
Santhoshkumar, S., Rajagopalsamy, C.B.T., Jawahar, P., Jayakumar, N. and Pavinkumar, P. (2017). Growth and mortality characteristics of Otolithes ruber (Schneider, 1801) exploited off Thoothukudi Coast, Tamil Nadu. J. Entomol. Zool., 5(4); 1746-1749.
Savari, S., Safahieh, A., Archangi, B., Savari, A. and Abdi, R. (2020). The histopathological effect of methylmercury on the brain in orange spotted grouper (Epinephelus coioides) in Zangi Creek and laboratory. Iran. J. Fish. Sci., 19(1); 457-470.
Savari, S., Safahieh, A., Archangi, B., Savari, A. and Abdi, R. (2016). Evaluation of acetylcholinesterase transcript level as a biomarker of methylmercury in orange spotted grouper (Epinephelus coioides) brain. Iran. J. Fish. Sci., 15(2); 898-912.
Sayed, A.H. and Hala, A.M. (2017). Melanomacrophage centers in Clarias gariepinus as immunological biomarker for toxicity of silver nanoparticles. J. Microsc. Ultrastruct., 5 (2); 97-104.
Sayed, A.H. and Hamed, H.S. (2017). Induction of apoptosis and DNA damage by 4-nonylphenol in African catfish (Clarias gariepinus) and the antioxidant role of Cydonia oblonga. Ecotoxicol. Environ. Saf., 139, 97-101.
Singh, A., Dar, M.Y., Joshi, B., Sharma, B., Shrivastava, S. and Shukla, S. (2018). Phytofabrication of silver nanoparticles: novel drug to overcome hepatocellular ailments. Toxicol. Rep., 5, 333-342.
Wu, Y. and Zhou, Q. (2013). Silver nanoparticles cause oxidative damage and histological changes in medaka (Oryzias latipes) after 14 days of exposure. Environ. Toxicol. Chem., 32, 165-173.