Effect of Biochar Amended Vermicomposting of Food and Beverage Industry Sludge along with Cow dung and Seed Germination Bioassay

Document Type : Original Research Paper


1 Department of Environmental Sciences, Jahangirnagar University, Dhaka-1342

2 Institute of National Analytical Research and Service (INARS), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh


Transformation of food and beverage industrial sludge into vermicompost into value-added product simultaneously can control gaseous emission. Addition of biochar in the vermicomposting as a bulking agent increases fertilizer value. This research aimed to investigate the effect of biochar amendment on vermicomposting of the food and beverage industry sludge (FBIS) and cow dung (CD) in a different ratio using earthworm Eisenia fetida. We had further investigated the survival rate of E. fetida and the cocoon productions after 35 days of the vermicomposting. Besides, we have also evaluated the seed germination bioassay using Malabar spinach (Basella alba) to determine the toxicity and maturity of produced compost. The survival and cocoon production of E. fetida were higher in vermicompost amended with 10% biochar. Vermicomposting with biochar resulted in a slight pH shift. Reduction in organic carbon (OC) percentage not so significant in biochar added FBIS and CD. An increase in phosphorus and potassium content and a decrease in nitrogen percentage observed; vermicomposting with biochar resulted in higher seed germination, root elongation, and germination index than vermicomposting without biochar.


APHA (American Public Health Association) (2012). Standard Methods for the Examination of Water and Wastewater. American Water Works Association, Water Environment Federation, USA, 22nd edition. Awasthi, M. K., Wang, Q., Huang, H., Li, R., Shen, F., Lahori, A. H., ... & Zhang, Z. (2016). Effect of biochar amendment on greenhouse gas emission and bio-availability of heavy metals during sewage sludge co-composting. J. Clean. Prod., 135, 829-835.
Bangladesh Biochar Initiative (2020) The Akha: "Agriculture-Friendly Stove". http://www.biochar-bangladesh.org/the-akha-agriculture-friendly-stove/ and TLUD-Biochar Ecology. http://www.biochar-bangladesh.org/cookstove-ecology/ Accessed 30 June 2020.
364 Tasnim et al.
Bhat, S. A., Singh, J. and Vig, A. P. (2018). Earthworms as organic waste managers and biofertilizer producers. Waste Biomass Valorization , 9(7), 1073-1086. Chen, Y. X., Huang, X. D., Han, Z. Y., Huang, X., Hu, B., Shi, D. Z. and Wu, W. X. (2010). Effects of bamboo charcoal and bamboo vinegar on nitrogen conservation and heavy metals immobility during pig manure composting. Chemosphere, 78(9), 1177-1181. Chowdhury, M. A., de Neergaard, A. and Jensen, L. S. (2014). Potential of aeration flow rate and bio-char addition to reduce greenhouse gas and ammonia emissions during manure composting. Chemosphere, 97, 16-25. Dias, B. O., Silva, C. A., Higashikawa, F. S., Roig, A. and Sánchez-Monedero, M. A. (2010). Use of biochar as bulking agent for the composting of poultry manure: effect on organic matter degradation and humification. Bioresour. Technol., 101(4), 1239-1246. Doan, T. T., Henry-des-Tureaux, T., Rumpel, C., Janeau, J. L. and Jouquet, P. (2015). Impact of compost, vermicompost and biochar on soil fertility, maize yield and soil erosion in Northern Vietnam: a three year mesocosm experiment. Sci. Total. Environ., 514, 147-154. Easha, N. J., Rahaman, M. S., Zaman, T. and Uddin, K. (2015). Feasibility study of vermicomposting of textile sludge mixed with cow dung and seed germination bioassay for toxicity evaluation of the produced compost. Int. J. Environ. Prot. Policy, 3, 27-34. Gong, X., Cai, L., Li, S., Chang, S. X., Sun, X. and An, Z. (2018). Bamboo biochar amendment improves the growth and reproduction of Eisenia fetida and the quality of green waste vermicompost. Ecotoxicol. Environ. Saf., 156, 197-204. Hua, L., Wu, W., Liu, Y., McBride, M. B. and Chen, Y. (2009). Reduction of nitrogen loss and Cu and Zn mobility during sludge composting with bamboo charcoal amendment. Environ. Sci. Pollut. Res., 16(1), 1-9. Huang, L., Gu, M., Yu, P., Zhou, C. and Liu, X. (2020). Biochar and Vermicompost Amendments Affect Substrate Properties and Plant Growth of Basil and Tomato. Agronomy, 10(2), 224. International Biochar Initiative (2014). Comparison of European Biochar Certificate Version 4. 8 and IBI Biochar Standards Version 2.0. https://www.biochar-international.org/wp-content/uploads/2018/04/IBI-EBC_comparison_Oct2014.pdf accessed 30 June 2020. Jadia, C. D. and Fulekar, M. H. (2008). Vermicomposting of vegetable waste: A biophysicochemical process based on hydro-operating bioreactor. Afr. J. Biotechnol., 7(20). Jindo, K., Sánchez-Monedero, M. A., Hernández, T., García, C., Furukawa, T., Matsumoto, K., ... & Bastida, F. (2012). Biochar influences the microbial community structure during manure composting with agricultural wastes. Sci. Total Environ., 416, 476-481. Kiyasudeen, K., Ibrahim, M. H., Quaik, S. and Ismail, S. A. (2016). Vermicomposting: An Earthworm Mediated Waste Treatment Technique. In Prospects of Organic Waste Management and the Significance of Earthworms, 167-199. Springer, Cham. Lv, B., Cui, Y., Wei, H., Chen, Q. and Zhang, D. (2020). Elucidating the role of earthworms in N2O emission and production pathway during vermicomposting of sewage sludge and rice straw. J. Hazard. Mater., 400, 123215. Malińska, K., Zabochnicka-Świątek, M. and Dach, J. (2014). Effects of biochar amendment on ammonia emission during composting of sewage sludge. Ecol. Eng., 71, 474-478. Malińska, K., Zabochnicka-Świątek, M., Cáceres, R. and Marfà, O. (2016). The effect of precomposted sewage sludge mixture amended with biochar on the growth and reproduction of Eisenia fetida during laboratory vermicomposting. Ecol. Eng., 90, 35-41. Méndez, A., Gómez, A., Paz-Ferreiro, J. and Gascó, G. (2012). Effects of sewage sludge biochar on plant metal availability after application to a Mediterranean soil. Chemosphere, 89(11), 1354-1359. Sánchez-García, M., Alburquerque, J. A., Sánchez-Monedero, M. A., Roig, A. and Cayuela, M. L. (2015). Biochar accelerates organic matter degradation and enhances N mineralisation during composting of poultry manure without a relevant impact on gas emissions. Bioresour. Technol., 192, 272-279.
Pollution 2021, 7(2): 355-365 365
Shams, S., Sahu, J. N., Rahman, S. S. and Ahsan, A. (2017). Sustainable waste management policy in Bangladesh for reduction of greenhouse gases. Sustain. Cities Soc., 33, 18-26. Singh, A., Karmegam, N., Singh, G. S., Bhadauria, T., Chang, S. W., Awasthi, M. K., ... & Ravindran, B. (2020). Earthworms and vermicompost: an eco-friendly approach for repaying nature’s debt. Environ Geochem Health, 1-26. Steiner, C., Das, K. C., Melear, N. and Lakly, D. (2010). Reducing nitrogen loss during poultry litter composting using biochar. J. Environ. Qual., 39(4), 1236-1242. Tam, N. F. Y. and Tiquia, S. (1994). Assessing toxicity of spent pig litter using a seed germination technique. Resour Conserv Recycl., 11(1-4), 261-274. Vandecasteele, B., Sinicco, T., D'Hose, T., Nest, T. V. and Mondini, C. (2016). Biochar amendment before or after composting affects compost quality and N losses, but not P plant uptake. J. Environ. Manage., 168, 200-209. Wu, S., He, H., Inthapanya, X., Yang, C., Lu, L., Zeng, G. and Han, Z. (2017). Role of biochar on composting of organic wastes and remediation of contaminated soils—a review. Environ. Sci. Pollut., 24(20), 16560-16577. Yadav, A. and Garg, V. K. (2009). Feasibility of nutrient recovery from industrial sludge by vermicomposting technology. J. Hazard. Mater., 168(1), 262-268. Yadav, A. and Garg, V. K. (2016). Vermiconversion of biogas plant slurry and parthenium weed mixture to manure. Int. j. recycl. org. waste agric., 5(4), 301-309. Zhang, J., Lü, F., Luo, C., Shao, L. and He, P. (2014). Humification characterization of biochar and its potential as a composting amendment. J Environ Sci., 26(2), 390-397. Zhang, L. and Sun, X. (2016). Influence of bulking agents on physical, chemical, and microbiological properties during the two-stage composting of green waste. Waste Manage., 48, 115-126. Zucconi, F. D. (1987). Compost specifications for the production and characterization of compost from municipal solid waste. Compost: production, quality and use, 30-50.